Table 2 Primer sequences used for PCR amplification and sequencing
Primer name | Primer sequence | Amplicon size (bp) | Sequencing primer |
|---|---|---|---|
mir-1-1F | 5′- gagatggattcagggatgga -3′ | 494 | mir-1-1R |
mir-1-1R | 5′- acctgctgacacaggcaaag -3′ |  |  |
mir-1-2F | 5′- ggaaccattaatgccatgct -3′ | 467 | mir-1-2F |
mir-1-2R | 5′- tgaaatctacttcactggatcttctt -3′ |  |  |
mir-133a-1F | 5′- tttaaaccattaagcgcagga -3′ | 455 | mir-133a-1F |
mir-133a-1R | 5′- ttgaaatccttaagtcatccataca -3′ |  |  |
mir-133a-2F | 5′- ctgcagagcttgagggaaac -3′ | 466 | mir-133a-2R |
mir-133a-2R | 5′- caaggaggaacaagcaggag -3′ |  |  |
mir-133bF | 5′- agtcatgcaacatgaaatacaaa -3′ | 500 | mir-133bR |
mir-133bR | 5′- gagtgcaaaggcacagaaca -3′ |  |  |