Immunodetection of components of landmark structures and FISH mapping of (TTAGGG)-repeat sites on lampbrush chromosome H of P. ridibundus (the lake frog). a, f. Identification of giant structures (giant fusing loops) and marker loops enriched with splicing factors. Immunofluorescent staining with antibodies K121 against TMG-cap of snRNA (a) and antibodies Y12 against Sm-proteins (f). c. Identification of chromosome associated nucleolus using immunofluorescent staining with antibodies No-185 against nucleolus protein Nopp-140. Dotted lines indicate marker structures. d. FISH mapping of (TTAGGG)n repeat on lampbrush chromosome H. Telomeres and interstitial (TTAGGG)-repeat sites were detected and shown by dotted lines. g. Detection of coilin-positive granules (indicated by dotted lines) by immunofluorescent staining with R288 antibody. Arrowheads show centromeres. Nu – extrachromosomal nucleoli. Chromosomes are counterstained with DAPI. Scale bars = 10 μm. b, e. Color indication of marker structures according to their molecular components on cytological map of lampbrush chromosome H. Loops accumulating splicing factors are colored green, chromosome associated nucleolus – blue, coilin accumulating granules – red.