Loss of imprinting in hiPSCs. Loss of allelic expression of GTL2 in cell line AM-iPS-7 (1) and PEG1 in cell lines UtE-iPS-6 and 11 (2). PCR products amplified from paired genomic DNA and cDNA samples were digested with the specified restriction enzymes (A). Results were confirmed by direct sequencing (B). AM936EP and UtE1104 are primary culture cells made from amniotic membrane and uterine endometrium cells, respectively. Band ‘a’: without a restriction site. Band ‘b’: with a restriction site.