Aberrant DNA methylation of hiPSCs with LOI. Bisulfite PCR sequencing methylation assay of genomic DNA prepared from AM-iPS-7 and MRC-iPS-25 at the IG-DMR (GTL2-DMR) (A) and UtE-iPS-6 and 11 at PEG1 (B). Each row represents a unique methylation profile within the pool of 20 clones sequenced. Closed and open circles represent methylated and unmethylated CpGs, respectively. The numbers represent the percentages of methylation by bisulfite sequencing. SNPs are shown by arrows.