Figure 4

Reactivation of GTL2 expression by treatment with VPA. Reactivation of GTL2 expression by treatment with VPA (A). The expression level of GTL2 mRNA was restored by VPA treatment. Gene expression of the original cells and of the hiPSCs was compared to that of hESCs. The GAPDH ratio was calculated. The bars indicate the means ± SD from two replicates. The imprinting status of GTL2 was stable in response to VPA (B). Methylation status in bisulfite-PCR sequencing analyses of IG-DMR is unchanged (C). Histone modifications of the GTL2 promoter were changed by VPA (D). The immunoprecipitation/input ratio was calculated. The bars indicate the means ± SD from three replicates.