Skip to main content
Figure 5 | BMC Genetics

Figure 5

From: Variants in the 3' UTR of General Transcription Factor IIF, polypeptide 2 affect female calving efficiency in Japanese Black cattle

Figure 5

Luciferase reporter assay for the 5' upstream region and the 3' UTR of GTF2F2 derived Q and q haplotypes. (A) Schematic representation of the positions of variants in the 5' upstream region and the 3' UTR of GTF2F2. “S” and “indel” represent SNPs and indel, respectively. The detailed positions of the variants are shown in Additional file 5. The 5' upstream region (2,964 bp upstream from the start codon, blue) and the 3' UTR of GTF2F2 (from 810 bp to 1,745 bp of mRNA, red) derived from the Q and q haplotypes were cloned into Firefly luciferase (pGL3-Q and pGL3-q) and Renilla luciferase (pRL-TK-Q and pRL-TK-q) vectors, respectively. The Firefly-to-Renilla luminescence ratios observed when cotransfecting HeLa cells (B) or bovine endometrial epithelial cells (D) with the indicated luciferase reporters were measured to evaluate the effects of the 5' upstream region (blue). The Renilla-to-Firefly luminescence ratios observed when cotransfecting HeLa cells (C) or bovine endometrial epithelial cells (E) with the indicated luciferase reporters were measured to evaluate the effects of the 3' UTR (red). Error bars represent the ± SEM obtained in triplicate from three experiments. P values determined by t-test are shown in the graph.

Back to article page