- Research article
- Open Access
Reintroductions and genetic introgression from domestic pigs have shaped the genetic population structure of Northwest European wild boar
© Goedbloed et al.; licensee BioMed Central Ltd. 2013
Received: 23 January 2013
Accepted: 10 May 2013
Published: 20 May 2013
Population genetic studies focus on natural dispersal and isolation by landscape barriers as the main drivers of genetic population structure. However, anthropogenic factors such as reintroductions, translocations and wild x domestic hybridization may also have strong effects on genetic population structure. In this study we genotyped 351 Single Nucleotide Polymorphism markers evenly spread across the genome in 645 wild boar (Sus scrofa) from Northwest Europe to evaluate determinants of genetic population structure.
We show that wild boar genetic population structure is influenced by historical reintroductions and by genetic introgression from domestic pigs. Six genetically distinct and geographically coherent wild boar clusters were identified in the Netherlands and Western Germany. The Dutch Veluwe cluster is known to be reintroduced, and three adjacent Dutch and German clusters are suspected to be a result of reintroduction, based on clustering results, low levels of heterozygosity and relatively high genetic distances to nearby populations. Recent wild x domestic hybrids were found geographically widespread across clusters and at low frequencies (average 3.9%). The relationship between pairwise kinship coefficients and geographic distance showed male-biased dispersal at the population genetic level.
Our results demonstrate that wildlife and landscape management by humans are shaping the genetic diversity of an iconic wildlife species. Historical reintroductions, translocation and recent restocking activities with farmed wild boar have all influenced wild boar genetic population structure. The current trend of wild boar population growth and range expansion has recently led to a number of contact zones between clusters, and further admixture between the different wild boar clusters is to be expected.
Most population genetic studies consider dispersal and isolation by landscape barriers to be the main drivers of genetic population structure . However, human activities such as reintroductions, translocations and genetic introgression from domestic sources, may play an important role in certain study systems, in addition to natural dispersal and landscape patterns [2–4]. Such human activities, legal or not, are often poorly documented and their population genetic effects are mostly unknown. Molecular techniques provide increasingly powerful and affordable tools to evaluate anthropogenic influences on wildlife genetic population structure [5, 6]. The use of Single Nucleotide Polymorphisms (SNPs) in particular is promising for the fields of population and conservation genetics [7, 8].
Wild boar became extinct in large parts of Western Europe in the 19th century . The species was marginalized mainly by overhunting and deforestation associated with increased agricultural land use. Extinction in Britain had already occurred in the 13th century . This massive decline in Western Europe was followed by an unknown number of mostly undocumented reintroductions in the late 19th and early 20th century. One such event is the commonly known but undocumented reintroduction of wild boar to the Veluwe, the forested centre of The Netherlands, which occurred in 1904 at the orders of Hendrik, Prince-Consort of Queen Wilhelmina of The Netherlands, for the purpose of hunting . These animals are thought to stem from Northeast Germany and Czech Republic.
Conditions for wild boar steadily improved during the 20th century due to hunting restrictions, reforestation, changes in agriculture and possibly climate change [12, 13]. Starting from 1960, wild boar populations throughout Europe saw rapid growth and range expansion [14, 15]. Wild boar (Sus scrofa) are adaptive and opportunistic omnivores as well as good dispersers, being able to travel distances up to 250 km  and fast breeders, with litter sizes of 4–7 once a year . Dispersal is male-biased in this species [9, 17]. European wild boar population structure at the continental scale is mainly shaped by post-glacial colonization patterns . It is, however, unknown how the history of marginalization, reintroductions and recent population expansion has affected the genetic population structure at local or regional scales. In an area such as The Netherlands and Western Germany, one could expect high rates of gene flow.
Wild boar farming became popular in Europe in the second half of the 20th century to provide for a demand in luxury meat. Hybridization between wild boar and domestic breeds is common practise on these farms to achieve increased reproduction and growth rates . Such hybrids have been shown to be the source of the escaped wild boar population in England . Introduction of wild boar originating from hybrid farmed stocks has also been shown in mainland Europe . This has effectively led to genetic introgression from domestic pigs into local wild boar populations. Recent hybrids (until 5th generation backcrosses with wild boar) as well as advanced generation hybrids (resulting from reproduction among hybrids across multiple generations) were identified. However, the spatial extent of domestic introgression and its effects on the population genetic structure of European wild boar have not been studied in detail.
From an evolutionary point of view, possible adverse effects of genetic introgression from a domestic or hybrid source include genetic adaptation to captivity and possibly outbreeding depression , while possible advantageous effects include hybrid vigour, increased growth rates and larger litter size. These evolutionary advantageous effects may be undesirable from a management perspective, as more rapidly reproducing wild boar can be difficult to control using normal population management practices and can then cause significant damage to agricultural crops . Strikingly high litter sizes and strong differences in litter size between regions have indeed been observed in wild boar in Germany . In addition to evolutionary effects, also population composition and structure can be affected by hybrid introductions and restocking practices .
In this study we used 351 SNP markers, genotyped for 645 wild boar, including 88 samples from a previous study , to assess the effects of historical marginalization, reintroductions and genetic introgression from domestic pigs on the population genetic structure of wild boar in The Netherlands and Western Germany.
Blood or tissue samples were taken from a total of 645 wild boar in parts of The Netherlands, Western Germany and Luxembourg. This included 88 samples from a previous study , which were genotyped using the Illumina porcine SNP60 genotyping beadchip . All samples were collected in the years 2008–2010 from animals identified in the field as wild boar. Sampling was performed on animals culled by wildlife managers for reasons of routine wildlife management or in the context of obligate disease monitoring programs. No animals were killed or inconvenienced for the purpose of this study. This study was approved a priori by Laboratory Animal Science officials in compliance with Dutch law.
DNA was extracted using the Qiagen PureGene (Blood) kit protocol. Samples were genotyped for 384 SNPs selected from the Illumina porcine SNP60 genotyping beadchip  from loci known to be polymorphic in wild boar in the study area, with proportional coverage of each chromosome and random selection within each chromosome. Of these 60k SNPs, 76% proved to be polymorphic in our wild boar dataset. Random selection within the autosomal and X chromosomes was performed to minimalize ascertainment bias. The only possible remaining ascertainment bias in our SNP set is derived from the ascertainment panel of the Illumina porcine SNP60 genotyping beadchip itself, and is considered to have no effect on the inference of wild boar population structure in the study area. Less than 0.0033% of the pairwise distances between the 351 randomly chosen SNPs were closer than 50,000 bp, which is considered to be the maximum range of physical linkage in wild boar . Selected SNPs were genotyped on an Illumina GoldenGate bead array platform (BeadXpress, Illumina Inc.) in a 96 well, 384 SNP format . Genotyping quality was assessed using GenomeStudio software (Illumina Inc.). Low genotyping quality or lack of differentiation between homozygote and heterozygote clusters lead to the removal of 33 SNPs. This left 351 non-coding SNPs for data analysis (Additional files 1 and 2), which is roughly equivalent in statistical power to 140 microsatellites [29, 30].
Linkage Disequilibrium (LD) was analysed in plink v1.06 by calculating all genome-wide pairwise SNP-SNP correlation coefficients (r2) and assuming a 0.2 threshold. Principal Components Analysis (PCA) was performed to visualise genetic variation and possible clustering patterns using the eigenvector method implemented in eigensoft 3.0 [32, 33]. For comparison, a sample of 120 domestic pigs from six breeds was used (Large White, Landrace, Duroc, Pietrain, British Saddleback and Tamworth, n = 20 per breed, Additional files 3 and 4). We used structure for population assignment analysis with 10 runs per number of clusters (K) for K = 1-10 with 500,000 iterations and a burnin of 800,000. Optimal partitioning was evaluated using the method proposed by Evanno et al.. Phylogenetic network analysis was performed using SplitsTree4 . A number of R packages were used: Adegenet  for heterozygosity calculations, Hierfstat  for calculation of FST values, SNPRelate  for the Maximum Likelihood Estimation calculation of kinship coefficients  based on the method of Thompson , and finally Vegan  for mantel tests in the Isolation By Distance (IBD) analysis, where genetic distance was calculated as FST/(1- FST) between all sampled locations.
The 351 genotyped SNPs had an overall call rate of 0.98 and 8 out of the 61075 possible pairwise SNP combinations (0.013%) interfered with linkage equilibrium. These pairwise LD SNP combinations were separated by 74-753 kb. As 50 kb is the maximum range of physical linkage in wild boar , the LD identified here must be caused by alternative mechanisms.
Results of hybrid detection using structure at different assignment thresholds
Total hybrids 1
Shared hybrids 2
SNP60 only 3
Type II error
structure only 4
Type I error
Detection of the nine previously studied SNP60 hybrid individuals at a structure assignment threshold of 0.25 (see Table 1 )
(type II error)
Genetic wild boar clusters with the corresponding sample size ( n ), observed heterozygosity (H o ) and number of hybrids
Autosomal F ST values between wild boar clusters (and domestic pigs)
Isolation by distance (IBD) analysis results for the full dataset and the different clusters separately
max. dist. (km)
Population genetic patterns and historical reintroductions
The largest wild boar populations in this study are found in Germany (West-Rhine and East-Rhine, Figure 4). They are relatively closely related (Table 4 and Figure 5) and most likely represent historically continuous wild boar populations. A high density of closely connected forest patches facilitates dispersal and genetic homogenisation in this part of the study area, and is only bisected by a natural barrier: the River Rhine (Figure 4). This barrier is not complete, as a few individuals seem to have crossed the Rhine in Germany. The barrier function of the River Rhine is, however, apparently sufficient to cause clear population differentiation between these clusters (FST =0.050, Table 4).
The wild boar found just South of the Rhine in the Netherlands, which belong to the Veluwe cluster (Figure 4), most likely represent an anthropogenic translocation event, as the intermediate terrain contains no forest and is intersected by two major rivers (the River Rhine and either the Waal or the IJssel). No wild boar were observed in this area until 1983.
The North-western section of the study area is characterised by a low level of fragmented forest cover, which is the main habitat for wild boar in Europe . Historical records show that substantial forest patches appeared in this part of the study area only after the advent of artificial fertilizers and its associated reduction of landscape-wide grazing pressure at the beginning of the 20th century . It is unlikely that wild boar occurred in the North-western part of the study area before 1900, due to a lack of suitable habitat (forest). One cluster (Veluwe) in this North-western section certainly originates from reintroductions in 1904, and the other three clusters (Meinweg, Hambach and Kirchhellen) most likely also arose from reintroductions in the 20th century. This is supported by clear genetic differentiation of each of these clusters (Table 4, Figures 1 and 5) with the other clusters, which may be explained by founder effects and subsequent reproductive isolation. The observed heterozygosity of these four populations is lower than in the Rhine populations (Table 3) supporting a historical population bottleneck or founder effect. The only exception is the Hambach cluster, which displays observed heterozygosity levels similar to the Rhine populations, but this may be explained by historical genetic introgression from domestic pigs, as discussed below. The absence of IBD in the (putatively) reintroduced populations: Veluwe, Meinweg, Hambach and Kirchhellen (Table 5), could be due to a history of introduction or translocation. On the other hand, absence of IBD may also be caused by a lack of statistical power due to small sample size (number of locations) and relatively small geographical range in these clusters. Wild boar from the Meinweg, Hambach and Kirchhellen are genetically well differentiated (Table 4, Figures 1, 3 and 5), even more so than the Veluwe cluster. The sources of the putative reintroductions in Meinweg, Hambach and Kirchhellen are unknown.
The Hambach cluster has a small geographical distribution with two localised foci (Figure 4). These two foci consist of small isolated forest patches, one of which is formed by a large brown coal mine in Germany (the Tagebau Hambach, opened in 1978, total surface 8500 hectare) and forested former refuse dump sites and fringes. This area was originally cleared of forest and only in 1980–1982 were the first dump sites (Sophienhohe) reforested, thereby creating opportunities for wild boar (re)colonisation. The other forest patch (Echt-Montfort, the Netherlands) was unoccupied by wild boar until 1983. Only one individual assigned by structure to the Hambach cluster (from the Echt-Montfort patch) was included in a previous SNP60 study . This individual was then identified as an advanced generation domestic-wild hybrid. Mitochondrial DNA haplotype analysis performed in that study revealed a typical domestic pig mitochondrial haplotype in this individual. The sudden appearance of this clearly distinct wild boar cluster in Hambach and in Echt-Montfort in the 1980s, together with the evidence of genetic influences from domestic pig suggest anthropogenic introduction, most likely from a captive wild boar source. A domestic hybrid origin or influence in this cluster would also explain the relatively high levels of observed heterozygosity in such a small population (Table 3).
We assume the three populations (putatively) reintroduced in the early 20th century (Veluwe, Meinweg and Kirchhellen) to have existed in complete reproductive isolation initially. However, wild boar populations across Europe have increased their numbers dramatically since the 1960s [9, 14, 15]. The contact zones between wild boar clusters found in this study based on the geographical overlap of clusters (e.g., Meinweg, Hambach and West-Rhine as well as Kirchhellen and East-Rhine, see Figure 4) are considered to be a consequence of these population expansions and therefore relatively recent. structure identified a relatively small number of admixed wild boar (Figure 1), all associated with contact zones. This low frequency of admixture supports a recent onset of contact between clusters.
Identification and effects of genetic introgression from domestic pigs
The mechanism for genetic introgression from domestic pigs into wild boar populations is most likely deliberate or accidental introduction of hybrid farmed wild boar [18, 21]. The structure algorithm identified 25 geographically scattered recent hybrids in 645 wild boar samples (3.9%). Hybrids are not more frequent in (putative) reintroduced populations, and seem to be recently introduced to various parts of the study area, possibly for the purpose of restocking local hunting grounds.
The structure algorithm relies solely on typical domestic pig allele frequencies for domestic-wild hybrid detection. Allele frequencies may change over time due to genetic drift and admixture with local wild boar gene pools. The figures based on hybrid detection by structure therefore only represent recent genetic introgression from domestic pigs and are likely to underestimate or disregard historical genetic introgression. Hybrid identification using a structure assignment threshold of 0.25 to the domestic pig cluster reliably identified all recent hybrids studied in a previous high-density SNP study , but not the advanced generation hybrids. This result indicates that allele frequency signatures from both source populations (wild and domestic) were indeed only detectable in relatively recent hybrids (approximately up to five generations of backcrossing) .
Phylogenetic analysis indicated multiple separate lineages within the hybrid group (Figure 5), suggesting that different hybridisation events are responsible for the detected genetic introgression from domestic pigs. This corresponds to previous findings from mtDNA haplotype analysis , which also suggested multiple origins of wild-domestic hybrids in this area.
If low numbers of hybrids are introduced in already occupied wild boar habitat, they would be expected to mate mostly with local wildtype individuals, leading to a rapid dilution of hybrid genetic signal over a few generations . However, if hybrids are to be introduced in areas previously unoccupied by wild boar, reproduction will occur mostly among hybrids. Over time this could lead to local dominance of advanced generation hybrids and a persistent hybrid genetic signal. Advanced generation hybrids such as those produced by the latter scenario would not be identifiable as being of partly domestic origin by structure, because allele frequencies are likely to have diverged over time from those of the source populations due to genetic drift and admixture with local wild boar gene pools. However, these hybrids should be detected when analysing Allele Frequency Spectrum Assessment, which is based on introgressed allelic states (e.g., ). Such a scenario of older hybridisation followed by introduction to the wild and reproduction among hybrids may have shaped the Hambach cluster.
Exclusion of recent hybrids from our total dataset resulted in an average population FST increase of 0.0093, corresponding to 8% of the average population FST (Table 4). This demonstrates that domestic introgression may affect the results of population differentiation analysis in certain study systems. Here, only recent hybrids (approximately up to fifth generation backcrosses) could be excluded. Long-term effects of domestic introgression most likely also exist (e.g., in the Hambach cluster), potentially affecting genetic population structure further. The LD found in this wild boar dataset is most likely also a consequence of recent genetic introgression, although effects of population substructure and small local population sizes could not be ruled out or corrected for. As a general recommendation for population genetic analysis, we propose that hybrid detection should be performed in all cases where genetic introgression is deemed possible, to avoid associated biases in population differentiation (FST) or LD, as well as erroneous interpretations of population structure.
The presence of six well-defined genetic clusters in the study area can be attributed to two factors: the presence of a natural barrier: the River Rhine, and a history of marginalization, extinction and subsequent anthropogenic reintroductions in the Northwest of the study area. Widespread genetic signatures of recent accidental or deliberate restocking of local populations with hybrid farmed wild boar have been found, which confounded population differentiation statistics, but which do not seem to affect the existing population structure.
In this study we demonstrate the effect of past landscape and population management on current population structure in an iconic wildlife species. Effects of historical deforestation and overhunting followed by reintroductions and restocking from farms are evident. Wild boar populations in the study area are currently expanding their range. Previously isolated populations are admixing in recently formed contact zones. The relative contribution of each of the current populations to future wild boar diversity may depend on a number of factors including the effective size of populations, habitat connectivity, founder effects, restocking activity, introductions and translocations.
We thank the Animal Health Service Deventer (GDD) in the Netherlands and dr. Karl Zimmer from the Landesuntersuchungsamt of Rhineland Palatinate in Germany for sample contributions. Thanks go to Bert Dibbits and Thijs Bosch for technical assistance. We appreciate comments on the manuscript by Robert H. Kraus. Finally we thank the Royal Dutch Hunters Association (KNJV) and De Eik Foundation for financial support.
- Manel S, Schwartz MK, Luikart G, Taberlet P: Landscape genetics: combining landscape ecology and population genetics. Trends Ecol Evol. 2003, 18 (4): 189-197. 10.1016/S0169-5347(03)00008-9.View ArticleGoogle Scholar
- Hartl GB, Zachos F, Nadlinger K: Genetic diversity in European red deer (cervus elaphus L.): anthropogenic influences on natural populations. Cr Biol. 2003, 326: S37-S42.View ArticleGoogle Scholar
- Pierpaoli M, Biro ZS, Herrmann M, Hupe K, Fernandes M, Ragni B, Szemethy L, Randi E: Genetic distinction of wildcat (felis silvestris) populations in Europe, and hybridization with domestic cats in Hungary. Mol Ecol. 2003, 12 (10): 2585-2598. 10.1046/j.1365-294X.2003.01939.x.View ArticlePubMedGoogle Scholar
- Vernesi C, Crestanello B, Pecchioli E, Tartari D, Caramelli D, Hauffe H, Bertorelle G: The genetic impact of demographic decline and reintroduction in the wild boar (Sus scrofa): a microsatellite analysis. Mol Ecol. 2003, 12 (3): 585-595. 10.1046/j.1365-294X.2003.01763.x.View ArticlePubMedGoogle Scholar
- Randi E: Detecting hybridization between wild species and their domesticated relatives. Mol Ecol. 2008, 17 (1): 285-293. 10.1111/j.1365-294X.2007.03417.x.View ArticlePubMedGoogle Scholar
- Frantz AC, Pourtois JT, Heuertz M, Schley L, Flamand MC, Krier A, Bertouille S, Chaumont F, Burke T: Genetic structure and assignment tests demonstrate illegal translocation of red deer (cervus elaphus) into a continuous population. Mol Ecol. 2006, 15 (11): 3191-3203. 10.1111/j.1365-294X.2006.03022.x.View ArticlePubMedGoogle Scholar
- Morin PA, Luikart G, Wayne RK: SNPs in ecology, evolution and conservation. Trends Ecol Evol. 2004, 19 (4): 208-216. 10.1016/j.tree.2004.01.009.View ArticleGoogle Scholar
- Willing EM, Bentzen P, van Oosterhout C, Hoffmann M, Cable J, Breden F, Weigel D, Dreyer C: Genome-wide single nucleotide polymorphisms reveal population history and adaptive divergence in wild guppies. Mol Ecol. 2010, 19 (5): 968-984. 10.1111/j.1365-294X.2010.04528.x.View ArticlePubMedGoogle Scholar
- Briedermann: Schwarzwild. 1990, Berlin, Germany: VEB Deutscher LandwirtschaftsverlagGoogle Scholar
- Yalden DW: The history of british mammals. 1999, London, UK: Poyser Natural HistoryGoogle Scholar
- Pelzers E: Beknopte historie van het wilde zwijn in Nederland. Zoogdier. 1990, 1 (1): 25-29.Google Scholar
- Bieber C, Ruf T: Population dynamics in wild boar Sus scrofa: ecology, elasticity of growth rate and implications for the management of pulsed resource consumers. J Appl Ecol. 2005, 42 (6): 1203-1213. 10.1111/j.1365-2664.2005.01094.x.View ArticleGoogle Scholar
- Prins HHT: The origins of grassland communities in northwestern europe. Grazing and conservation management. Edited by: WallisDeVries MF, Bakker JP, Van Wieren SE. 1998, Boston: Kluwer Academic Publishers, 55-105.View ArticleGoogle Scholar
- Geisser H, Reyer HU: The influence of food and temperature on population density of wild boar Sus scrofa in the Thurgau (Switzerland). J Zool. 2005, 267: 89-96. 10.1017/S095283690500734X.View ArticleGoogle Scholar
- Saez-Royuela C, Telleria JL: The increased population of the wild boar (Sus-scrofa L) in europe. Mammal Rev. 1986, 16 (2): 97-101. 10.1111/j.1365-2907.1986.tb00027.x.View ArticleGoogle Scholar
- Andrzejewski R, Jezierski W: Management of a wild boar population and its effects on commercial land. Acta Theriol. 1978, 23 (19–3): 309-339.View ArticleGoogle Scholar
- Truve J, Lemel J: Timing and distance of natal dispersal for wild boar Sus scrofa in Sweden. Wildlife Biol. 2003, 9: 51-57.Google Scholar
- Scandura M, Iacolina L, Apollonio M: Genetic diversity in the European wild boar Sus scrofa: phylogeography, population structure and wild x domestic hybridization. Mammal Rev. 2011, 41 (2): 125-137. 10.1111/j.1365-2907.2010.00182.x.View ArticleGoogle Scholar
- Booth WD: Wild boar farming in the United Kingdom. IBEX J Mt Ecol. 1995, 3: 245-248.Google Scholar
- Frantz AC, Massei G, Burke T: Genetic evidence for past hybridization between domestic pigs and english wild boars. Conserv Genet. 2012, 13 (5): 1355-1364. 10.1007/s10592-012-0379-1.View ArticleGoogle Scholar
- Goedbloed DJ, Megens HJ, van Hooft P, Herrero-Medrano JM, Lutz W, Panoraia A, Crooijmans RP, Groenen MA, van Wieren SE, Ydenberg RC: Genome-wide single nucleotide polymorphism reveals recent genetic introgression from domestic pigs into Northwest European wild boar populations. Mol Ecol. 2013, 22 (3): 856-866. 10.1111/j.1365-294X.2012.05670.x.View ArticlePubMedGoogle Scholar
- Frankham R: Quantitative genetics in conservation biology. Genet Res. 1999, 74 (3): 237-244. 10.1017/S001667239900405X.View ArticlePubMedGoogle Scholar
- Schley L, Dufrene M, Krier A, Frantz AC: Patterns of crop damage by wild boar (Sus scrofa) in Luxembourg over a 10-year period. Eur J Wildl Res. 2008, 54 (4): 589-599. 10.1007/s10344-008-0183-x.View ArticleGoogle Scholar
- Gethoffer F, Sodeikat G, Pohlmeyer K: Reproductive parameters of wild boar (Sus scrofa) in three different parts of germany. Eur J Wildl Res. 2007, 53 (4): 287-297. 10.1007/s10344-007-0097-z.View ArticleGoogle Scholar
- Laikre L, Schwartz MK, Waples RS, Ryman N, Grp GW: Compromising genetic diversity in the wild: unmonitored large-scale release of plants and animals. Trends Ecol Evol. 2010, 25 (9): 520-529. 10.1016/j.tree.2010.06.013.View ArticlePubMedGoogle Scholar
- Ramos AM, Crooijmans RPMA, Affara NA, Amaral AJ, Archibald AL: Design of a high-density SNP genotyping assay in the pig using SNPs identified and characterized by next generation sequencing technology. Plos 1. 2009, 4 (8):Google Scholar
- Amaral AJ, Megens HJ, Crooijmans RPMA, Heuven HCM, Groenen MAM: Linkage disequilibrium decay and haplotype block structure in the pig. Genetics. 2008, 179 (1): 569-579. 10.1534/genetics.107.084277.PubMed CentralView ArticlePubMedGoogle Scholar
- Fan JB, Gunderson KL, Bibikova M, Yeakley JM, Chen J, Garcia EW, Lebruska LL, Laurent M, Shen R, Barker D: Illumina universal bead arrays. DNA microarrays part a: array platforms and Wet-bench protocols. vol. 410. 2006, San Diego: Elsevier Academic Press Inc, 57-+-View ArticleGoogle Scholar
- Herraez DL, Schafer H, Mosner J, Fries HR, Wink M: Comparison of microsatellite and single nucleotide polymorphism markers for the genetic analysis of a galloway cattle population. Z Naturforsch C. 2005, 60 (7–8): 637-643.Google Scholar
- Schopen GCB, Bovenhuis H, Visker MHPW, van Arendonk JAM: Comparison of information content for microsatellites and SNPs in poultry and cattle. Anim Genet. 2008, 39 (4): 451-453. 10.1111/j.1365-2052.2008.01736.x.View ArticlePubMedGoogle Scholar
- Purcell S, Neale B, Todd-Brown K, Thomas L, Ferreira MA, Bender D, Maller J, Sklar P, de Bakker PI, Daly MJ: PLINK: a tool set for whole-genome association and population-based linkage analyses. Am J Hum Genet. 2007, 81 (3): 559-575. 10.1086/519795.PubMed CentralView ArticlePubMedGoogle Scholar
- Patterson N, Price AL, Reich D: Population structure and eigenanalysis. PLoS Genet. 2006, 2 (12): e190-10.1371/journal.pgen.0020190.PubMed CentralView ArticlePubMedGoogle Scholar
- Price AL, Patterson NJ, Plenge RM, Weinblatt ME, Shadick NA, Reich D: Principal components analysis corrects for stratification in genome-wide association studies. Nat Genet. 2006, 38 (8): 904-909. 10.1038/ng1847.View ArticlePubMedGoogle Scholar
- Pritchard JK, Stephens M, Donnelly P: Inference of population structure using multilocus genotype data. Genetics. 2000, 155 (2): 945-959.PubMed CentralPubMedGoogle Scholar
- Evanno G, Regnaut S, Goudet J: Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol. 2005, 14 (8): 2611-2620. 10.1111/j.1365-294X.2005.02553.x.View ArticlePubMedGoogle Scholar
- Huson DH, Bryant D: Application of phylogenetic networks in evolutionary studies. Mol Biol Evol. 2006, 23 (2): 254-267.View ArticlePubMedGoogle Scholar
- Jombart T: Adegenet: a R package for the multivariate analysis of genetic markers. Bioinformatics. 2008, 24 (11): 1403-1405. 10.1093/bioinformatics/btn129.View ArticlePubMedGoogle Scholar
- Goudet J: HIERFSTAT, a package for R to compute and test hierarchical F-statistics. Mol Ecol Notes. 2005, 5 (1): 184-186. 10.1111/j.1471-8286.2004.00828.x.View ArticleGoogle Scholar
- Zheng X: SNPRelate: parrallel computing toolset for genome-wide association studies. R package version 095. 2012Google Scholar
- Milligan BG: Maximum-likelihood estimation of relatedness. Genetics. 2003, 163 (3): 1153-1167.PubMed CentralPubMedGoogle Scholar
- Thompson EA: Estimation of pairwise relationships. Ann Hum Genet. 1975, 39 (Oct): 173-188.View ArticlePubMedGoogle Scholar
- Oksanen J, Blanchet GF, Kindt R, Legendre P, Minchin PR, O'Hara RB, Simpson GL, Solymos P, Stevens MHH, Wagner H: Vegan: community ecology package. R package version 20–3. 2012Google Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.