Table 1 New SSR loci developed for Paspalum
Locus name | Repeat motif | Ta(°C) | Product size(bp) | NM | PIC | F(5´-3´) | R(5´-3´) | GenBank accession number |
|---|---|---|---|---|---|---|---|---|
PN02-B3 | (GT)12 | 50 | 139-157 | 11 | 0,8 | CAAACAATGGGACAACACTT | TTTTTCTGCTCTGGCTCAT | HQ585421 |
PN02-C6 | (TG)8 | 51,4 | 139-145 | 4 | 0,3 | TTCAGCAATCAAGAGTTAGA | AGAGACGATCAGGGTGTG | HQ585422 |
PN02-G3 | (GT)9 | 60 | 294-302 | 5 | 0,6 | GTCGCCGGTCGCAGTCG | CACATAGCCGGCCTCCTCTC | HQ585423 |
PN02-G7 | (AC)7 | TOUCHDOWN | 118 | 7 | 0,7 | TGCAGCATGTAATAACC | TACACAGGAAGGAAGAAA | HQ585424 |
PN03-C7 | (GT)6. | 50 | 159-165 | 3 | 0,6 | TCATGCCACGAGAAAGAAACCA | CAGGGACGACAGGGACAAGACT | HQ585425 |
PN03-D10 | (GT)11 | 50 | 280-300 | 7 | 0,7 | GTTATTCCTTCACTCACTCACC | AGCTTTCTGCCTTCTTTTT | HQ585426 |
PN03-D12 | (AG)10 | 50 | 220-240 | 7 | 0,7 | TGGGGAAGCAGGAAGTCACA | CAGCTCACCGATGGGAATG | HQ585427 |
PN03-E7 | (GT)9 | TOUCHDOWN | 298 | 7 | 0,7 | CATGCTTTTCTGCTTCC | GCTAATACTGCTTGCTCTTC | HQ585428 |
PN03-F1 | (CA)7 | 50 | 184-192 | 5 | 0,7 | CAGTGACCAAGCTTACAACCTA | GTGGCATGCCTTTCTACAA | HQ585429 |
PN03-F10 | (TG)7 | 60 | 165-177 | 7 | 0,8 | TGCCTACCGTTTCCTCTTCTCT | GGGATGGGTCTTGACTCTTGAT | HQ585433 |
PN03-F3 | (GT)8 | 60 | 165-203 | 8 | 0,8 | ACCCCGCGTCCTCTTTCTCA | CCCAACCCACCATTATCCTCTG | HQ585430 |
PN03-F7 | (CA)10 | 50 | 200-212 | 6 | 0,8 | TGCTGGCAGTCTTTCTCT | GGCCATACCACTTTTTG | HQ585431 |
PN03-F9 | (CA)8 | 60 | 179-275 | 15 | 0,9 | AAAAGGGCAGGAGTTAGGAC | AGTTGGCTCAGTTACGATTTTA | HQ585432 |
PN03-G5 | (CA)7 | 60 | 126-138 | 6 | 0,7 | AAGTCACCGTTTCAATCCAG | GCAGTGCGCAAGTTTTCT | HQ585434 |
PN03-H3 | (GA)22 | 51,4 | 198-250 | 11 | 0,8 | CATGTAAACGTAGCAGAGGT | GCTAATTTTACAGGGTGGTC | HQ585435 |
PA01-C8 | (AT)2TTA(AT)2TTCA(GA)2 | 60 | 247-279 | 6 | 0,8 | AATCTGACCTGTTTTACTTCTC | TGCATTTTTGGGATACACT | HM208140 |
PA01-E10 | (GGT)2 T(ACT)2 | 54 | 186-210 | 6 | 0,4 | GCGATTATTGTTGTTTGGTTTG | ACGCTTTCTTGTCTTCATCTCA | HM208141 |
Average | Â | Â | Â | 7,1 | 0,7 | Â | Â | Â |