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Figure 4 | BMC Genetics

Figure 4

From: Splice variants and promoter methylation status of the Bovine Vasa Homology (Bvh) gene may be involved in bull spermatogenesis

Figure 4

Alternative splice of Bvh in bovine testes. A. Agarose gel electropherograms of the splice variants from testis tissues developed by RT-PCR. Line 1-3 were amplification fragment of primer 1, line1 was full-length transcript, and line 2-3 were splice variants. Line 4 was amplification fragment of primer 2. Molecular marker was DL2000. B. Splice variants in coding region of Bvh. Number labelled boxes indicate the genomic regions identical to cDNA (exon2 to 6). FL means the full-length transcript, V4 is splice variant lack of exon4 and V45 is splice variant lack of exon4 and 5. C. Amino acids changes caused by alternative splicing. The alignment figures display the changes at the protein level of two identified splice variants compared to full-length protein.

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