Developing molecular tools and insights into the Penstemon genome using genomic reduction and next-generation sequencing

Table 2 The four multiplex identifiers (MID) barcodes (adapter) primers used for the genomes of Penstemon cyananthus , P. dissectus , P. davidsonii , and P. fruticosus

Species	MID ID #	*Eco*R1 MID primer¹	*Bfa*1 MID primer²
P. cyananthus	MID 1	5′- ACGAGTGCGTGACTGCGTACCAATTC	5′- ACGAGTGCGTGATGAGTCCTGAGTA
P. dissectus	MID 2	5′- ACGCTCGACAGACTGCGTACCAATTC	5′- ACGCTCGACAGATGAGTCCTGAGTA
P. davidsonii	MID 3	5′- AGACGCACTCGACTGCGTACCAATTC	5′- AGACGCACTCGATGAGTCCTGAGTA
P. fruticosus	MID 4	5′- AGCACTGTAGGACTGCGTACCAATTC	5′- AGCACTGTAGGATGAGTCCTGAGTA

¹ The “AATTC” at the 3′ end the primer was where adapters complement the enzyme EcoR1 cut site and the preceding “C” is where the base was changed to avoid further enzymatic cleavage of the fragment.
² The “TA” at the 3′ end of the primer was where adapters complement the enzyme Bfa1 cut site and the preceding “G” is where the base was changed to avoid further enzymatic cleavage of the fragment.

ISSN: 2730-6844