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Table 4 Summary data from 454-pyrosequencing and Newbler de-novo assembly (v.2.0.01) of Penstemon cyananthus , P. dissectus , P. davidsonii , and P. fruticosus

From: Developing molecular tools and insights into the Penstemon genome using genomic reduction and next-generation sequencing

Assembly

Genome size (Mbp)1

GC content

Reads

Bases2

% Reads assembled

% Bases assembled

Contigs created

Bases in assembly

% Genome represented

Average coverage

Bases shared between assemblies

P. cyananthus

893

36.4%

199,329

87,753,792

53.1%

50.0%

9,714

4,623,755

0.5%

7.7X

 

P. dissectus

462

34.5%

98,868

43,304,550

52.8%

50.9%

5,364

2,629,819

0.6%

8.2X

 

P. davidsonii

483

35.3%

103,963

45,599,742

45.8%

43.5%

4,882

2,376,141

0.5%

9.1X

 

P. fruticosus

476

35.2%

113,146

49,786,980

41.0%

38.8%

4,777

2,322,606

0.5%

8.9X

 

P. cyananthus × P. dissectus

  

298,197

131,058,342

53.0%

50.1%

14,523

6,915,079

  

338,495

P. cyananthus × P. davidsonii

  

303,292

133,353,534

49.9%

46.9%

14,254

6,757,023

  

242,873

P. cyananthus × P. fruticosus

  

312,475

137,540,772

47.8%

44.9%

14,134

6,705,536

  

240,825

P. dissectus × P. davidsonii

  

202,831

88,904,292

48.3%

46.1%

10,053

4,855,491

  

150,469

P. dissectus × P. fruticosus

  

212,014

93,091,530

45.7%

43.5%

9,873

4,774,539

  

177,886

P. davidsonii × P. fruticosus

  

217,109

95,386,722

44.0%

41.7%

9,184

4,442,194

  

256,553

Full Penstemon Assembly

  

730,215

265,987,500

47.9%

46.4%

44,966

16,363,589

   
  1. 1 The diploid (2n = 2x = 16) genome size as reported by Broderick et al. [5].
  2. 2 Bases denotes the total number of bases used to create the assembly and not the total number of bases sequenced.