Giemsa-stained karyotypes and chromosome pairs sequentially submitted the Ag-NOR method. (A) L11- São Luís/MA (ZUEC 16216, male), (B) L12- Bacabeira/MA (ZUEC 16225, male), (C) L13- Urbano Santos/MA (ZUEC 13665, female), (D) L14-Porto Nacional/TO (ZUEC 13491, male), (E) L15- Barreiras/BA with four different specimens (ZUEC 17075, male; ZUEC 17072, male; ZUEC 17071, male and ZUEC 17082, male) showing additional secondary constrictions and NOR sites and (F) L16-Uberlandia/MG (ZUEC 15888, male). The arrows indicate the secondary constrictions. Insets (right) show NOR-bearing chromosomes and arrowheads indicate NOR sites. Bar=3 μm.