Skip to main content

Table 3 Virtual restriction mapping of 3.56 Kb unit of DYZ1 array from twin pairs (blood DNA) using restriction mapper software

From: DYZ1 arrays show sequence variation between the monozygotic males

Sr. no. Restriction site Sequence Overhang Restriction site frequency
MZT1a MZT1b MZT2a MZT2b MZT3a MZT3b
1 Cac8I GCNNGC 5’ 1 1 1 1 2 1
2 BseYI CCCAGC 5’ 1 1 1 1 1 1
3 Bsp1407I TGTACA 5’ 1 1 1 1 0 0
4 BspHI TCATGA 3’ 1 1 1 1 0 0
5 ClaI ATCGAT 3’ 1 1 1 1 1 1
6 DdeI CTNAG blunt 1 1 0 1 0 0
7 EcoRI GAATTC 5’ 1 1 1 1 2 1
8 ScrFI CCNGG 3’ 1 1 1 1 1 1
9 VspI ATTAAT blunt 1 1 1 1 1 1
10 BseMII CTCAG 5’ 1 1 0 1 0 0
11 BsgI GTGCAG 3’ 1 1 1 1 0 0
12 BtsI GCAGTG 3’ 1 1 1 1 0 0
13 Eco57I CTGAAG 3’ 1 1 1 1 1 1
14 GsuI CTGGAG 3’ 1 1 1 1 0 0
15 Hpy188I TCNGA 3’ 1 1 1 1 0 0
16 MnlI CCTC 3’ 1 1 2 2 0 0
17 PflMI CCANNNNNTGG 3’ 1 1 1 1 1 1
18 SduI GDGCHC blunt 1 1 1 1 0 0
19 BsaBI GATNNNNATC blunt 0 0 1 0 0 0
20 TaqII GACCGA 3’ 0 0 2 0 0 0
21 MfeI CAATTG 5’ 0 0 0 2 0 0
22 DpnI GATC 5’ 2 2 1 1 1 1
23 FokI GGATG 3’ 2 2 2 2 2 2
24 MboI GATC 5’ 2 2 1 1 1 1
25 ArsI GACNNNNNNTTYG 5’ 2 2 0 2 2 2
26 BdaI TGANNNNNNTCA 5’ 2 2 2 2 2 2
27 CspCI CAANNNNNGTGG 5’ 2 0 0 0 0 0
28 Eco57MI CTGRAG 5’ 2 2 2 2 1 1
29 SetI ASST 5’ 2 2 2 2 2 1
30 TspRI CASTG 5’ 2 2 2 2 1 1
31 ApoI RAATTY 3’ 3 3 3 3 4 2
32 AsuII TTCGAA 3’ 3 3 3 3 3 4
33 Eco31I GGTCTC blunt 3 3 3 3 3 3
34 TatI WGTACW 5’ 3 3 3 4 1 1
35 MboII GAAGA 3’ 3 4 4 3 3 4
36 CviJI RGCY blunt 4 4 4 4 3 3
37 MslI CAYNNNNRTG 5’ 4 4 4 4 3 3
38 BccI CCATC 3’ 4 4 5 4 4 5
39 MaeI CTAG 3’ 4 4 4 4 3 4
40 SfaNI GCATC 3’ 4 4 3 4 2 2
41 AlfI GCANNNNNNTGC 3’ 4 4 2 4 2 2
42 TstI CACNNNNNNTCC 3’ 4 4 4 5 4 4
43 RsaI GTAC 3’ 5 5 5 5 2 3
44 BsmAI GTCTC 3’ 5 5 5 5 5 5
45 BstXI CCANNNNNNTGG blunt 5 4 5 5 5 5
46 XmnI GAANNNNTTC 5’ 6 6 6 6 5 6
47 MseI TTAA 3’ 6 6 6 5 6 6
48 NlaIII CATG 5’ 7 7 9 7 5 4
49 BcgI CGANNNNNNTGC 5’ 14 14 18 16 10 12
50 BsrDI GCAATG 5’ 15 14 14 16 11 11
51 BsrI ACTGG 5’ 19 18 19 18 12 14
52 TspGWI ACGGA 5’ 19 19 21 19 16 16
53 BsmI GAATGC 5’ 21 20 22 22 13 17
54 AgsI TTSAA 5’ 22 22 24 25 24 20
55 FaiI YATR 3’ 23 23 25 23 17 16
56 PleI GAGTC 3’ 23 23 24 23 19 18
57 TspDTI ATGAA blunt 25 25 23 24 18 17
58 TspEI AATT 5’ 34 32 36 34 32 29
59 TfiI GAWTC 3’ 53 53 51 54 48 48
60 TaqI TCGA blunt 66 67 61 67 54 55
61 HinfI GANTC 5’ 76 76 75 77 67 66
  1. The differences are highlighted in bold.