Table 3 Virtual restriction mapping of 3.56 Kb unit of DYZ1 array from twin pairs (blood DNA) using restriction mapper software
From: DYZ1 arrays show sequence variation between the monozygotic males
Sr. no. | Restriction site | Sequence | Overhang | Restriction site frequency | |||||
|---|---|---|---|---|---|---|---|---|---|
MZT1a | MZT1b | MZT2a | MZT2b | MZT3a | MZT3b | ||||
1 | Cac8I | GCNNGC | 5’ | 1 | 1 | 1 | 1 | 2 | 1 |
2 | BseYI | CCCAGC | 5’ | 1 | 1 | 1 | 1 | 1 | 1 |
3 | Bsp1407I | TGTACA | 5’ | 1 | 1 | 1 | 1 | 0 | 0 |
4 | BspHI | TCATGA | 3’ | 1 | 1 | 1 | 1 | 0 | 0 |
5 | ClaI | ATCGAT | 3’ | 1 | 1 | 1 | 1 | 1 | 1 |
6 | DdeI | CTNAG | blunt | 1 | 1 | 0 | 1 | 0 | 0 |
7 | EcoRI | GAATTC | 5’ | 1 | 1 | 1 | 1 | 2 | 1 |
8 | ScrFI | CCNGG | 3’ | 1 | 1 | 1 | 1 | 1 | 1 |
9 | VspI | ATTAAT | blunt | 1 | 1 | 1 | 1 | 1 | 1 |
10 | BseMII | CTCAG | 5’ | 1 | 1 | 0 | 1 | 0 | 0 |
11 | BsgI | GTGCAG | 3’ | 1 | 1 | 1 | 1 | 0 | 0 |
12 | BtsI | GCAGTG | 3’ | 1 | 1 | 1 | 1 | 0 | 0 |
13 | Eco57I | CTGAAG | 3’ | 1 | 1 | 1 | 1 | 1 | 1 |
14 | GsuI | CTGGAG | 3’ | 1 | 1 | 1 | 1 | 0 | 0 |
15 | Hpy188I | TCNGA | 3’ | 1 | 1 | 1 | 1 | 0 | 0 |
16 | MnlI | CCTC | 3’ | 1 | 1 | 2 | 2 | 0 | 0 |
17 | PflMI | CCANNNNNTGG | 3’ | 1 | 1 | 1 | 1 | 1 | 1 |
18 | SduI | GDGCHC | blunt | 1 | 1 | 1 | 1 | 0 | 0 |
19 | BsaBI | GATNNNNATC | blunt | 0 | 0 | 1 | 0 | 0 | 0 |
20 | TaqII | GACCGA | 3’ | 0 | 0 | 2 | 0 | 0 | 0 |
21 | MfeI | CAATTG | 5’ | 0 | 0 | 0 | 2 | 0 | 0 |
22 | DpnI | GATC | 5’ | 2 | 2 | 1 | 1 | 1 | 1 |
23 | FokI | GGATG | 3’ | 2 | 2 | 2 | 2 | 2 | 2 |
24 | MboI | GATC | 5’ | 2 | 2 | 1 | 1 | 1 | 1 |
25 | ArsI | GACNNNNNNTTYG | 5’ | 2 | 2 | 0 | 2 | 2 | 2 |
26 | BdaI | TGANNNNNNTCA | 5’ | 2 | 2 | 2 | 2 | 2 | 2 |
27 | CspCI | CAANNNNNGTGG | 5’ | 2 | 0 | 0 | 0 | 0 | 0 |
28 | Eco57MI | CTGRAG | 5’ | 2 | 2 | 2 | 2 | 1 | 1 |
29 | SetI | ASST | 5’ | 2 | 2 | 2 | 2 | 2 | 1 |
30 | TspRI | CASTG | 5’ | 2 | 2 | 2 | 2 | 1 | 1 |
31 | ApoI | RAATTY | 3’ | 3 | 3 | 3 | 3 | 4 | 2 |
32 | AsuII | TTCGAA | 3’ | 3 | 3 | 3 | 3 | 3 | 4 |
33 | Eco31I | GGTCTC | blunt | 3 | 3 | 3 | 3 | 3 | 3 |
34 | TatI | WGTACW | 5’ | 3 | 3 | 3 | 4 | 1 | 1 |
35 | MboII | GAAGA | 3’ | 3 | 4 | 4 | 3 | 3 | 4 |
36 | CviJI | RGCY | blunt | 4 | 4 | 4 | 4 | 3 | 3 |
37 | MslI | CAYNNNNRTG | 5’ | 4 | 4 | 4 | 4 | 3 | 3 |
38 | BccI | CCATC | 3’ | 4 | 4 | 5 | 4 | 4 | 5 |
39 | MaeI | CTAG | 3’ | 4 | 4 | 4 | 4 | 3 | 4 |
40 | SfaNI | GCATC | 3’ | 4 | 4 | 3 | 4 | 2 | 2 |
41 | AlfI | GCANNNNNNTGC | 3’ | 4 | 4 | 2 | 4 | 2 | 2 |
42 | TstI | CACNNNNNNTCC | 3’ | 4 | 4 | 4 | 5 | 4 | 4 |
43 | RsaI | GTAC | 3’ | 5 | 5 | 5 | 5 | 2 | 3 |
44 | BsmAI | GTCTC | 3’ | 5 | 5 | 5 | 5 | 5 | 5 |
45 | BstXI | CCANNNNNNTGG | blunt | 5 | 4 | 5 | 5 | 5 | 5 |
46 | XmnI | GAANNNNTTC | 5’ | 6 | 6 | 6 | 6 | 5 | 6 |
47 | MseI | TTAA | 3’ | 6 | 6 | 6 | 5 | 6 | 6 |
48 | NlaIII | CATG | 5’ | 7 | 7 | 9 | 7 | 5 | 4 |
49 | BcgI | CGANNNNNNTGC | 5’ | 14 | 14 | 18 | 16 | 10 | 12 |
50 | BsrDI | GCAATG | 5’ | 15 | 14 | 14 | 16 | 11 | 11 |
51 | BsrI | ACTGG | 5’ | 19 | 18 | 19 | 18 | 12 | 14 |
52 | TspGWI | ACGGA | 5’ | 19 | 19 | 21 | 19 | 16 | 16 |
53 | BsmI | GAATGC | 5’ | 21 | 20 | 22 | 22 | 13 | 17 |
54 | AgsI | TTSAA | 5’ | 22 | 22 | 24 | 25 | 24 | 20 |
55 | FaiI | YATR | 3’ | 23 | 23 | 25 | 23 | 17 | 16 |
56 | PleI | GAGTC | 3’ | 23 | 23 | 24 | 23 | 19 | 18 |
57 | TspDTI | ATGAA | blunt | 25 | 25 | 23 | 24 | 18 | 17 |
58 | TspEI | AATT | 5’ | 34 | 32 | 36 | 34 | 32 | 29 |
59 | TfiI | GAWTC | 3’ | 53 | 53 | 51 | 54 | 48 | 48 |
60 | TaqI | TCGA | blunt | 66 | 67 | 61 | 67 | 54 | 55 |
61 | HinfI | GANTC | 5’ | 76 | 76 | 75 | 77 | 67 | 66 |