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Table 4 Virtual restriction mapping of 3.56 kb unit of DYZ1 array from MZT1 (both blood and germline DNA) using Restriction Mapper software

From: DYZ1 arrays show sequence variation between the monozygotic males

Sr. No. Restriction Site Sequence Overhang Restriction Enzyme Site Frequency
     MZT1a (Blood) MZT1a (Germline) MZT1b (Blood) MZT1b (Germline)
1 Cac8I GCNNGC 5’ 1 1 1 1
2 BseYI CCCAGC 5’ 1 1 1 1
3 Bsp1407I TGTACA 5’ 1 1 1 1
4 BspHI TCATGA 3’ 1 1 1 1
5 ClaI ATCGAT 3’ 1 1 1 1
6 DdeI CTNAG blunt 1 1 1 1
7 EcoRI GAATTC 5’ 1 1 1 1
8 ScrFI CCNGG 3’ 1 1 1 1
9 VspI ATTAAT blunt 1 2 1 2
10 BseMII CTCAG 5’ 1 1 1 1
11 BsgI GTGCAG 3’ 1 1 1 1
12 BtsI GCAGTG 3’ 1 1 1 1
13 Eco57I CTGAAG 3’ 1 1 1 1
14 GsuI CTGGAG 3’ 1 1 1 1
15 Hpy188I TCNGA 3’ 1 1 1 1
16 MnlI CCTC 3’ 1 1 1 1
17 PflMI CCANNNNNTGG 3’ 1 1 1 1
18 SduI GDGCHC blunt 1 1 1 1
19 AcyI GRCGYC 5’ 0 0 0 1
20 HgaI GACGC 5’ 0 0 0 1
21 Hpy99I CGWCG 3’ 0 0 0 1
22 HaeIV GAYNNNNNRTC 3’ 0 0 0 2
23 Hin4I GAYNNNNNVTC 3’ 0 0 0 2
24 DpnI GATC 5’ 2 2 2 3
25 FokI GGATG 3’ 2 2 2 3
26 MboI GATC 5’ 2 2 2 3
27 ArsI GACNNNNNNTTYG 5’ 2 2 2 2
28 BdaI TGANNNNNNTCA 5’ 2 2 2 2
29 CspCI CAANNNNNGTGG 5’ 2 0 0 0
30 Eco57MI CTGRAG 5’ 2 2 2 2
31 SetI ASST 5’ 2 3 2 2
32 TspRI CASTG 5’ 2 2 2 3
33 ApoI RAATTY 3’ 3 3 3 3
34 AsuII TTCGAA 3’ 3 4 3 3
35 Eco31I GGTCTC blunt 3 3 3 3
36 TatI WGTACW 5’ 3 3 3 3
37 MboII GAAGA 3’ 3 3 4 4
38 CviJI RGCY blunt 4 4 4 4
39 MslI CAYNNNNRTG 5’ 4 4 4 4
40 BccI CCATC 3’ 4 4 4 4
41 MaeI CTAG 3’ 4 4 4 3
42 SfaNI GCATC 3’ 4 4 4 5
43 AlfI GCANNNNNNTGC 3’ 4 4 4 4
44 TstI CACNNNNNNTCC 3’ 4 4 4 4
45 RsaI GTAC 3’ 5 5 5 5
46 BsmAI GTCTC 3’ 5 5 5 5
47 BstXI CCANNNNNNTGG blunt 5 5 4 5
48 XmnI GAANNNNTTC 5’ 6 6 6 6
49 MseI TTAA 3’ 6 6 6 6
50 NlaIII CATG 5’ 7 7 7 7
51 BcgI CGANNNNNNTGC 5’ 14 12 14 14
52 BsrDI GCAATG 5’ 15 14 14 15
53 BsrI ACTGG 5’ 19 19 18 19
54 TspGWI ACGGA 5’ 19 19 19 19
55 BsmI GAATGC 5’ 21 20 20 20
56 AgsI TTSAA 5’ 22 23 22 23
57 FaiI YATR 3’ 23 23 23 24
58 PleI GAGTC 3’ 23 24 23 24
59 TspDTI ATGAA blunt 25 24 25 24
60 TspEI AATT 5’ 34 35 32 35
61 TfiI GAWTC 3’ 53 52 53 51
62 TaqI TCGA blunt 66 65 67 66
63 HinfI GANTC 5’ 76 76 76 75
  1. The germline samples for remaining two twin pairs (MZT2 and 3) were not available. The differences in restriction site frequency are highlighted in bold.