Figure 3

Example of MSAP electropherograms documenting assessment of (-, +) MH patterns using Msp I + Hpa II double digestion. Fragments 1 and 3 represent the (-, +) MH pattern, fragment 2 represents the (+, +) MH pattern, and fragment 4 represents the (+, -) MH pattern. Note that fragments 1 and 3 disappear after application of the combination of the HpaII, MspI and EcoRI enzymes (M + H panel) or after DNA hypomethylation (H_h panel) by 100 μM DHPA [48]. Each restriction fragment is represented by two peaks (green and blue) derived from two complementary DNA strands (differently labeled selective primers were used). The presence of both peaks at the same position or in close proximity (note that the mobility of complementary strands of a DNA fragment may not be equal; [23]) indicates that the fragment had both EcoRI and MspI/HpaII ends.