Table 1 PCR primer sequences for methylation analysis of PTPRO
Primers | Sequences | Product size |
|---|---|---|
PTPRO-forward | 5′-CTCCACCCAAATCACTCTTCGCAG-3′ | 268 bp |
PTPRO-reverse | 5′-ACCATTGTTGAGACGGCTATGAACG-3′ | |
18 s rRNA-forward | 5′-TCAAGAACGAAAGTCGGAGG-3′ | 110 bp |
18 s rRNA- reverse | 5′-GGACATCTAAGGGCATCACA-3′ | |
MSP-methylated-forward | 5′-CGTTTTTGGAGGATTTCGGGC-3′ | 170 bp |
MSP-methylated- reverse | 5′-AAAACACGACTACGCTAACG-3′ | |
MSP-unmethylated-forward | 5′-ATGTTTTTGGAGGATTTTGGGT-3′ | 201 bp |
MSP-unmethylated- reverse | 5′-ATACCCCATCACTACACAAACA-3′ |  |