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Figure 1 | BMC Genetics

Figure 1

From: Karyotypic diversification in Mytilus mussels (Bivalvia: Mytilidae) inferred from chromosomal mapping of rRNA and histone gene clusters

Figure 1

Mapping of rDNA and histone gene clusters to the chromosomes of four species of Mytilus counterstained with DAPI. Double color FISH experiments using major (28S, green) and minor (5S, red) rDNA probes (a , d , g , j) show that major rDNA map to two chromosome pairs in M. edulis (a), M. galloprovincialis (d) and M. californianus (j) but to three in M. trossulus (g). All species show two separated 5S rDNA clusters on the biggest chromosome pair ( a , d , g , j ), however, two additional 5S rDNA loci are present in M. edulis (a), M. galloprovincialis (d) and M. trossulus (g). Two color FISH experiments using core (H3, red) and linker (H1L + H1C, green) histone gene probes show signals on four chromosome pairs in all Mytilus (b , e , h , k); two of the pairs bear signals corresponding to core histone genes and the other two to linker histone genes. Double color FISH experiments using two different linker histone gene probes, one for the h1 genes linked to the 5S rDNA (H1L, red) and the other for those constituting independent clusters (H1C, green) map to different chromosome pairs in all mussels (c , f , i , l). Scale bars, 5 μm.

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