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Figure 4 | BMC Genetics

Figure 4

From: Subtractive and differential hybridization molecular analyses of Ceratitis capitata XX/XY versus XX embryos to search for male-specific early transcribed genes

Figure 4

Molecular analysis of the BA11 clone. A) Schematic representation of alignments between the BA11 clone and the three corresponding Medfly ESTs. Percentages of nucleotide identities are reported. Red arrows indicate the position of the primers utilized. B) Genomic PCR and RT-PCR product analyses on adult male and female samples with Cc114+/BA11- primers pair. C) Genomic PCR and RT-PCR analyses on adult male and female samples with CcBA11+/CcBA11- primers pair. D) Genomic PCR and RT-PCR analyses on adult male and female samples with Cc483+/483-1- primer pair. E) Schematic representation of alignments between the PCR fragments amplified with Cc483+/CcBA11- primer pair. In light grey alignments between CcGen1 and CcGen2 clones and in dark grey alignments between CcGen2 and CcDmale clones. Percentages of nucleotide identities are also reported. Red arrows indicate the position of the primers utilized. F) Genomic PCR and RT-PCR analyses on adult male and female samples with Cc483+/CcBA11- primer pair, which led to identify a Y-linked paralog.

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