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Figure 1 | BMC Genetics

Figure 1

From: Male-specific phosphorylated SR proteins in adult flies of the Mediterranean Fruitfly Ceratitis capitata

Figure 1

SDS-PAGE electropherograms (A-B-C) and immunoblots (D-E-F-G-H) produced with protein extracts from adult males and females of Drosophila, M. domestica and C. capitata. 1) total lysate of males; 2) total lysate of females; 3) 90% ammonium sulfate precipitation supernatant of males; 4) 90% ammonium sulfate precipitation supernatant of females; 5) Mg++ supernatant of males; 6) Mg++ supernatant of females; 7) Mg++ pellets of males; 8) Mg++ pellets of females; P-) Mg++ pellets of C. capitata males without phosphatase treatment; P+) Mg++ pellets of C. capitata males with phosphatase treatment. After transfer to nitrocellulose, blots were incubated with either mAb104 or no primary antibody (as a control; data not shown).

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