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Table 2 Expression profiles of the 20 sex-determination (A) and three cellularisation (B) transcripts identified in the B. jarvisi CLC assembly. Expression in each sample BJ1 - BJ8 is reported as the normalised RPKM value from the comparison of early samples (BJ5-8) to late samples (BJ1-4). Also refer to Additional File 5.

From: Comprehensive transcriptome analysis of early male and female Bactrocera jarvisi embryos

     "early" samples "late" samples Significant up or down-regulation Comparison Validated expression profile#
     male female male female   
A. Sex determination genes contig no. contig size ORF size BJ5 BJ6 BJ7 BJ8 BJ1 BJ2 BJ3 BJ4 early vs late early vs late (exclude BJ1 and BJ5) Bj Cc Dm
doublesex F consensus 1839 321aa 0.42 0.19 0.17 0.22 0.55 0.04 0.09 0.03 ns ns M M Z
daughterless 16154 4053 710aa 3.03 3.39 9.88 9.94 1.51 4.52 4.26 4.53 ns ns    M
deadpan 20953 3365 574aa 0.28 0.25 0.26 1.06 0.95 10.92 11.18 24.97 ns 0.02   Z Z
degringolade 2208 1737 385aa 111.44 116.21 107.56 104.73 149.23 100.76 93.48 100.61 ns ns    M
extra macrochaetae 10611 1230 265aa 0.97 0.44 0.56 2.5 6.82 121.25 123.56 211.04 ns <0.001    M
female lethal d 2854 2694 466aa * 18.97 13.9 12.22 11.07 17.85 40.43 43.89 37.66 0.03 <0.001   M M
fruitless 29049 3175 675aa 0.78 0.57 0.74 0.43 0.62 0.19 0.38 0.08 ns ns    
groucho 3466 4604 726aa 16.34 7.42 13.32 12.69 13.69 51.44 59.52 94.36 ns <0.001   M M
hopscotch 753 4443 1174aa 53.5 20.8 23.27 19.15 40.31 15 20.17 23.18 ns ns    
Mes-4 5091 5397 1557aa 57.2 17.96 22.76 16.62 34.22 11.54 18.9 17.46 ns ns    
ovarian tumor consensus 5276 1221aa * 31.52 22.68 62.4 46.29 22.6 20.46 26.52 16.5 ns ns    
ovo (variant1) 21175 255 86aa * 3.93 2.97 8.77 14.62 1.44 0.09 0.43 0.23 ns ns    
ovo (variant 6) consensus 3076 739aa 12.66 11.2 58.05 51.86 5.85 0.83 0.61 0.86 ns ns    
runt consensus 2399 485aa 0.74 0.22 0.13 5.4 11.8 65.22 58.82 36.12 0.04 <0.001    Z
sans fille 7153 1421 150aa 35.59 80.26 75.06 85.98 43.71 65.1 55.76 50.27 ns 0.008   M  
scute consensus 817 272aa * 0.11 0.15 0.12 0.17 0.07 0.05 0.05 0.08 ns ns    Z
Sex-lethal 2265 1667 444aa * 23.93 21.11 23.7 22.69 22.68 38.45 42.67 64.18 ns 0.04 M M Z
sisterless A 2508 264 89aa * 71.62 39.09 29.59 38.58 89.42 180.25 216.18 294.05 0.03 <0.001   Z Z
transformer F consensus 3793 422aa 27.3 22.76 18.43 18.49 28.18 17.12 17.29 21.22 ns ns M M M
transformer-2 138 3165 251aa 48.16 44.99 41.74 44.01 47.47 37.15 37.52 39.39 ns ns M M M
virilizer consensus 5999 1878aa 27.4 31.59 38.11 36.09 30.89 28.63 32.69 27.83 ns ns    Z
B. Cellularisation genes                 
nullo consensus 1332 211aa 1.15 2.52 0.13 15.34 8.59 162.77 125.03 45.74 ns 0.04    Z
serendipity α 9105 5007 615aa * 13.31 11.32 10.66 9.64 12.42 4.22 3.5 4.31 ns ns   Z Z
slow as molasses 3824 5366 1568aa 2.02 0.76 0.25 13.35 35.87 460.96 425.02 299.29 ns <0.001 Z Z Z
  1. *Partial ORF
  2. #Experimentally validated in B. jarvisi (Bj), C. capitata (Cc) and D. melanogaster (Dm); maternal (M) or zygotic (Z) expression