Comprehensive transcriptome analysis of early male and female Bactrocera jarvisi embryos

Morrow, Jennifer L; Riegler, Markus; Gilchrist, A Stuart; Shearman, Deborah CA; Frommer, Marianne

doi:10.1186/1471-2156-15-S2-S7

BMC Genomic Data

Table 2 Expression profiles of the 20 sex-determination (A) and three cellularisation (B) transcripts identified in the B. jarvisi CLC assembly. Expression in each sample BJ1 - BJ8 is reported as the normalised RPKM value from the comparison of early samples (BJ5-8) to late samples (BJ1-4). Also refer to Additional File 5.

From: Comprehensive transcriptome analysis of early male and female Bactrocera jarvisi embryos

				"early" samples				"late" samples				Significant up or down-regulation Comparison		Validated expression profile#
				male		female		male		female
A. Sex determination genes	contig no.	contig size	ORF size	BJ5	BJ6	BJ7	BJ8	BJ1	BJ2	BJ3	BJ4	early vs late	early vs late (exclude BJ1 and BJ5)	Bj	Cc	Dm
doublesex F	consensus	1839	321aa	0.42	0.19	0.17	0.22	0.55	0.04	0.09	0.03	ns	ns	M	M	Z
daughterless	16154	4053	710aa	3.03	3.39	9.88	9.94	1.51	4.52	4.26	4.53	ns	ns			M
deadpan	20953	3365	574aa	0.28	0.25	0.26	1.06	0.95	10.92	11.18	24.97	ns	0.02		Z	Z
degringolade	2208	1737	385aa	111.44	116.21	107.56	104.73	149.23	100.76	93.48	100.61	ns	ns			M
extra macrochaetae	10611	1230	265aa	0.97	0.44	0.56	2.5	6.82	121.25	123.56	211.04	ns	<0.001			M
female lethal d	2854	2694	466aa *	18.97	13.9	12.22	11.07	17.85	40.43	43.89	37.66	0.03	<0.001		M	M
fruitless	29049	3175	675aa	0.78	0.57	0.74	0.43	0.62	0.19	0.38	0.08	ns	ns
groucho	3466	4604	726aa	16.34	7.42	13.32	12.69	13.69	51.44	59.52	94.36	ns	<0.001		M	M
hopscotch	753	4443	1174aa	53.5	20.8	23.27	19.15	40.31	15	20.17	23.18	ns	ns
Mes-4	5091	5397	1557aa	57.2	17.96	22.76	16.62	34.22	11.54	18.9	17.46	ns	ns
ovarian tumor	consensus	5276	1221aa *	31.52	22.68	62.4	46.29	22.6	20.46	26.52	16.5	ns	ns
ovo (variant1)	21175	255	86aa *	3.93	2.97	8.77	14.62	1.44	0.09	0.43	0.23	ns	ns
ovo (variant 6)	consensus	3076	739aa	12.66	11.2	58.05	51.86	5.85	0.83	0.61	0.86	ns	ns
runt	consensus	2399	485aa	0.74	0.22	0.13	5.4	11.8	65.22	58.82	36.12	0.04	<0.001			Z
sans fille	7153	1421	150aa	35.59	80.26	75.06	85.98	43.71	65.1	55.76	50.27	ns	0.008		M
scute	consensus	817	272aa *	0.11	0.15	0.12	0.17	0.07	0.05	0.05	0.08	ns	ns			Z
Sex-lethal	2265	1667	444aa *	23.93	21.11	23.7	22.69	22.68	38.45	42.67	64.18	ns	0.04	M	M	Z
sisterless A	2508	264	89aa *	71.62	39.09	29.59	38.58	89.42	180.25	216.18	294.05	0.03	<0.001		Z	Z
transformer F	consensus	3793	422aa	27.3	22.76	18.43	18.49	28.18	17.12	17.29	21.22	ns	ns	M	M	M
transformer-2	138	3165	251aa	48.16	44.99	41.74	44.01	47.47	37.15	37.52	39.39	ns	ns	M	M	M
virilizer	consensus	5999	1878aa	27.4	31.59	38.11	36.09	30.89	28.63	32.69	27.83	ns	ns			Z
B. Cellularisation genes
nullo	consensus	1332	211aa	1.15	2.52	0.13	15.34	8.59	162.77	125.03	45.74	ns	0.04			Z
serendipity α	9105	5007	615aa *	13.31	11.32	10.66	9.64	12.42	4.22	3.5	4.31	ns	ns		Z	Z
slow as molasses	3824	5366	1568aa	2.02	0.76	0.25	13.35	35.87	460.96	425.02	299.29	ns	<0.001	Z	Z	Z

*Partial ORF
#Experimentally validated in B. jarvisi (Bj), C. capitata (Cc) and D. melanogaster (Dm); maternal (M) or zygotic (Z) expression

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