Figure 3

Restriction fragment and sequence analysis of parental and rescued plasmids (A): The progenitor plasmid (pACT-HYG) and two aberrant rescued plasmids (RP8, RP9) were digested with the restriction enzymes indicated and the products visualised by agarose gel electrophoresis. The resulting fragment sizes from each reaction are given in Kb. (B): The rescued plasmid RP8 was sequenced using the M13 forward and reverse primer sites. Data shown in yellow (1–86; 629–650) represent cloning vector sequences with the Xho I site used to linearise the DNA prior to plasmid rescue underlined in bold uppercase. Data shown in blue (87–628) represent novel sequence not found in the progenitor plasmid pACT-HYG and derived either from the host cell bacterial genome or the Mos20 mosquito cell line genome.