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Figure 5 | BMC Genetics

Figure 5

From: Gene targeting in mosquito cells: a demonstration of 'knockout' technology in extrachromosomal gene arrays

Figure 5

Southern analysis of DNA from the AH-4 and HT-1 cell lines High molecular weight DNA from the AH-4 and HT-1 cell lines (5 μg per lane) was digested separately with Bam HI (Lanes B); Eco RI (Lanes E); Nco I (Lanes N) or Sph I (Lanes S) and fractionated on 0.75% agarose alongside undigested DNA (Lanes U). The DNA was transferred to a nitrocellulose membrane and hybridised sequentially with the hygromycin resistance gene (A) and the neomycin resistance gene (B). Hybridisation took place overnight at 42°C with membranes washed at high stringency (0.1 × SSC; 0.1% SDS; 60°C) and exposed to X-ray film against an intensifying screen for 5 hours at -70°C.

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