Table 3 Primers for amplification ofRAB27B coding regions
From: Chromosomal mapping, gene structure and characterization of the human and murine RAB27B gene
RAB27B | PCR fragment | Sense primer | Antisense primer |
|---|---|---|---|
exon (no.) | size (bp) | Â | Â |
2 | 252 | 5'-actttaatcaaaacatacttgttttcc | 5'-gggatgagtgacaaggttagaagtacg |
3 | 192 | 5'-tgagtgctgacaaaatccaaagcaacc | 5'-agtaagtttttctaagctatgtgtagc |
4 | 209 | 5'-tgtggactaactttcagaactaccc | 5'-aacaagcacctaaagcagttgagt |
5 | 237 | 5'-tttatcaataacttgctggttccat | 5'-atctaatgtctctgctccatttgt |
6 | 252 | 5'-tcaaaaatatttgccatgctttcta | 5'-gggttcttgatgttcagtttctatg |