Comparative analysis of recovered luciferase activities across cell lines. Mean luciferase activities (log10 cpm) recovered from the various transfections in cell lines derived from Ae. aegypti (Mos20; blue), An. gambiae (Ag55; magenta), Culex pipiens pallens (Cpp512, yellow), An. stephensi (As43, green) and An. sinensis ovary (Anso, red). Standard deviations are not shown for reasons of clarity but were comparable to those shown in Fig. 2 for An. gambiae (Ag55) cells. Transfections involved either circular (C) or linear (L) forms of the parent plasmid (p[ACT-LUC]) and deletion substrates (DL; DR), as shown, with superscripts indicating linearization by relevant restriction enzyme (LB, Bam HI; LS, Sac I; LH, Hin dIII; LSc, Sca I). A logarithmic scale was employed so that all values could be represented on the same figure.