Figure 2
From: NRG1 is required for glucose repression of the SUC2 and GAL genes of Saccharomyces cerevisiae
Deletion of NRG1 partially abolishes glucose repression. nrg1Δ allows cells to grow on sucrose plates containing 2-deoxyglucose, and has additive effects with mig1Δ and mig2Δ. A single colony of each strain was inoculated into liquid YPD and grown to saturation (approx. 1 × 108 cells/ml). The cultures were then diluted 1:2 (upper panels) or 1:5 (lower panels) in sterile water, and spotted on YPD plates and YP sucrose plates with 200 μg/ml 2-deoxyglucose. Plates were photographed on after 1 and 2 days of incubation at 30°C.