Deletion of NRG1 partially abolishes glucose repression. nrg1Δ allows cells to grow on sucrose plates containing 2-deoxyglucose, and has additive effects with mig1Δ and mig2Δ. A single colony of each strain was inoculated into liquid YPD and grown to saturation (approx. 1 × 108 cells/ml). The cultures were then diluted 1:2 (upper panels) or 1:5 (lower panels) in sterile water, and spotted on YPD plates and YP sucrose plates with 200 μg/ml 2-deoxyglucose. Plates were photographed on after 1 and 2 days of incubation at 30°C.