Figure 3
From: NRG1 is required for glucose repression of the SUC2 and GAL genes of Saccharomyces cerevisiae
Deletion of NRG1 causes defects in glucose repression. (A) A single colony of each strain was inoculated into YPD liquid with 2% glucose and grown to mid-log phase (approx. 1 × 107 cells/ml). The cells were harvested, and total RNA was isolated and analyzed by electrophoresis followed by hybridization with probes specific to SUC2 or SPT15. The intensities of each band was quantitated using phosphoimager and ImageQuant software. The amount of SUC2 mRNA in each strain was normalized to SPT15, and the result obtained for the wild-type strain was assigned the arbitrary unit of 1.0 and used to calculate the relative SUC2 mRNA levels in other strains. (B) Northern analysis of GAL1-10 mRNA in mutant strains. A single colony of each strain was inoculated into SD complete liquid with 2% glucose+2% galactose and grown to mid-log phase. The cells were harvested, and total RNA was isolated from each and analyzed by electrophoresis followed by hybridization with probes specific to GAL1, GAL10 or SPT15. Quantitation was carried out as for (A).