Isolation and characterization of new Saccharomyces cerevisiae mutants perturbed in nuclear pore complex assembly

Ryan, Kathryn J; Wente, Susan R

doi:10.1186/1471-2156-3-17

Table 1 Yeast Strains

From: Isolation and characterization of new Saccharomyces cerevisiae mutants perturbed in nuclear pore complex assembly

Strain	Genotype	Source
SWY519	MATα ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1::ADE2:ura3	Bucci and Wente, 1997
SWY1695	MAT a ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3	Bucci and Wente, 1998
SWY2089	MATα lys2 ura3-1 his3-11,15 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 nup170-GFP:URA3	this study
SWY2090	MAT a ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 nup170-GFP:URA3	this study
SWY2324	MATα sec13-G176R (npa2-1) lys2 ura3-1 his3-11,15 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 nup170-GFP:URA3	backcross of npa2-1 × SWY2090
SWY2325	MATα sec23-S383L (npa1-1) lys2 ura3-1 his3-11,15 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 nup170-GFP:URA3	backcross of npa1-1 × SWY2090
SWY2326	MATα sec6-4 lys2 ura3-1 his3-11,15 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 nup170-GFP:URA3	haploid from SWY2089 × NY17
SWY2327	MATα sec12-4 lys2 ura3-1 his3-11,15 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 nup170-GFP:URA3	haploid from SWY2090 × CKY39
SWY2328	MATα sec16-2 ura3-1 his3-11,15 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 nup170-GFP:URA3	haploid from SWY2089 × RSY268
SWY2329	MAT a sar1::URA3 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 nup170-GFP:URA3 p [GAL1-SAR1 LEU2]	haploid from SWY2090 × ANY27
SWY2330	MAT a sec31-1 ura3-1 his3-11,15 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 nup170-GFP:URA3	haploid from SWY2090 × RSY1004
SWY2331	MATα sec24 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 nup170-GFP:URA3	haploid from SWY2090 × CKY496
SWY2332	MATα sec27(npa3-1) ura3-1 his3-11,15 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 nup170-GFP:URA3	backcross of npa3-1 × SWY202089
SWY2333	MAT a bet3(npa10) ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 nup170-GFP:URA3	backcross of npa10 × SWY2089
SWY2334	MATα np4312 lys2 ura3-1 his3-11,15 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 nup170-GFP:URA3	original isolate from screen
SWY2335	MATα npa12 lys2 ura3-1 his3-11,15 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 nup170-GFP:URA3	original isolate from screen
SWY2336	MATα npa67 lys2 ura3-1 his3-11,15 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 nup170-GFP:URA3	original isolate from screen
YCH128	MATα lys2 ura3-1 leu2-3,112 his3-11,15 ade2-1 ade3	C. Hardy
ANY27	MATα sar1::URA3 ura3 trp1 leu2 his3 his4 p [GAL1-SAR1 LEU2]	Yamanushi et al., 1996
NY17	MAT a ura3-52 sec6-4	Potenza et al., 1992
CKY39	MATα ura3-52 his4-619 sec12-4	Kurihara et al., 2000
CKY496	MATα ura3-52 leu2-3,112 sec24-1	Kurihara et al., 2000
RSY268	MAT a ura3-52 sec16-2	Kaiser and Schekman, 1990
RSY1004	MATα ura3-52 leu2-3,112 sec31-1	Salama et al., 1997

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