Comparison of the human TDH gene sequences in the region of exon 4 and 6 acceptor sites with those of other species. (A) The splice donor and acceptor sites are indicated (gt and ag respectively). There is a splice site mutation in exon 4 in one of the human genomic sequences (g
g, coloured red). The translations of the genes are shown below the DNA sequences (ORF). The species and accession numbers are: Homo sapiens, HsTDH_gDNA_A (AF131216); HsTDH_gDNA_B (AC011959); Mus musculus, MmTDHgDNA (14.54000001-55000000 supercontig, Sanger Centre, UK) and puffer fish, Takifugu rubripes, TrTDH_gDNA (AF411956). (B) There is an in-frame stop codon (TGA, coloured blue) and a splice site mutation in exon 6 in the human genomic sequence (AF131216). A cryptic splice site (ag, coloured red) was utilised in cDNA clone 1 that altered the reading frame, resulting in another stop codon (TGA, coloured green). The species and accession numbers are: pig, Sus scrofa, SsTDH_cDNA (AY095535); MmTDH_cDNA (AY116662); rat, Rattus norvegicus, RmTDH_EST (BF557448); chicken, Gallus gallus, GgTDH_EST (BM491791); western clawed frog, Silurana tropicalis, StTDH_EST (AL638064); zebrafish, Danio rerio, DrTDH_ESTs; the tunicate, Ciona intestinalis, CiTDH_ESTs; fruit fly, Drosophila melanogaster, DmTDH_gDNA (AAF51607); the nematode, Caenorhabditis elegans, CeTDH_ESTs and the euglenoid, Trypanosoma brucei, TbTDH_gDNA (AC084047). There are no introns in this region of the fly, nematode and trypanosome genes (__). Identical nucleotides and residues are shown by *, strongly similar residues by: and weakly similar residues by (.).