The human homologue of unc-93 maps to chromosome 6q27 – characterisation and analysis in sporadic epithelial ovarian cancer

Table 2 Primers for amplification of UNC93A genomic DNA.

EXON	PRIMER NAME	PRIMER SEQUENCE (5'-3')	PCR PRODUCT SIZE (BP)	ANNEALING TEMPERATURE AND MG⁺⁺	IN DHPLC DENATURING TEMPERATURE (°C)	IN DHPLC GRADIENT START (INITIAL Buffer B%)
1	1f	GAATGGGACTTCTTGGTACTGATTG	225	61° C, 1 mM	59	52
	1r	TTCAGGTAATTTGATGACCGGACACA
2	2f	CTGTGCTCTGACAGGGCTGGCTTTGT	244	62° C, 1.5 mM	65	52
	2r	GGGTGGGCAGGGGGTGGTGGCTGCT
3	3f	CTATGGGTCTGCATTTTACCC	282	61° C, 1.5 mM	61	54
	3r	ACAATTGCTTGCCCCTTTTCCTTT
4	4f	ATTTGCCGTCATCTCATGTCT	195	61° C, 2 mM	-	-
	4r	CTGCCCTGGGCCGATGGAGCTCGT
5	5f	TGAAAGCTGAAGCCTTTGCTATGT	276	61° C, 1.5 mM	60	55
	5r	GAGCCACCGGCCCTCACTTTCGTT
6	6f	GCCTGGGAGCGTCCATGACGTGGC	204	61° C, 1.5 mM	-	-
	6r	GGATGGGACCCACGCTGATACCTA
7	7f	TCCCCTCTGCACCCCCAGGCGCGG	167	61° C, 2 mM	-	-
	7r	CTGGGCTGGGGACTCACCATTGTT
8 a	8f	TCACTCCGCTCTCTCCTCTGCAGC	166	58° C, 1.5 mM	-	-
	8r2	GACCCCCAGCAGAATGTAGAGCTT
8b	8f2	AAGCTCTACATTCTGCTGGGGGTC	173	58° C, 1.5 mM	-	-
	8r	TTCATCCTCCTCGGACCTCACTGCTCT
8	8f	TCACTCCGCTCTCTCCTCTGCAGC	315	62° C, 1 mM	63	55
	8r	TTCATCCTCCTCGGACCTCACTGCTCT

ISSN: 2730-6844