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Figure 1 | BMC Genetics

Figure 1

From: The Tnfrh1 (Tnfrsf23) gene is weakly imprinted in several organs and expressed at the trophoblast-decidua interface

Figure 1

Genes and PCR strategies. A, Map showing the positions of genes in the telomeric half of the distal mouse Chr7 imprinted domain. Direction of transcription is shown by the arrows, and imprinting is color-coded, with red indicating preferential expression of the maternal allele and blue indicating expression of the paternal allele. The Obph1 gene is coded as orange, since it shows an allelic expression bias only in the placenta, which may indicate either imprinting or expression from maternal cells. Genes that do not show an allelic expression bias, or for which complete information is not available, are in black. The KvDMR1 differentially methylated sequence, that acts as an imprinting control element and gives rise to the Kcnq1ot1 non-translated RNA, is indicated by the green bar. B, Structure of the Tnfrh1 and Tnfrh2 genes. PCR primers (arrows) are shown above the exons (rectangles). The yellow and orange shading highlights the non-conserved first exons. The last exons are highly conserved, and the remaining exons show intermediate conservation. Genetic polymorphisms in Tnfrh1 are indicated in violet (polymorphisms that distinguish CAST from BL/6) or maroon (polymorphisms that distinguish MOLD from BL/6). For analysis of allele-specific mRNA expression, we used primer pairs (1,2) or, alternatively (1,3). Estimation of relative expression from Tnfrh1 vs. Tnfrh2 by RT-PCR was done with primers 1, 4 and 5. Primers 1 and 6 were used to generate a Tnfrh1-specific cDNA probe.

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