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Figure 1 | BMC Genetics

Figure 1

From: Multiple telophase arrest bypassed (tab) mutants alleviate the essential requirement for Cdc15 in exit from mitosis in S. cerevisiae

Figure 1

Post-translational control of Clb2 proteolysis by CDC15. Exponentially growing cdc15-2 (RJD619) cells were arrested in late anaphase by shifting the culture to 37°C for three hours. The culture was split in three and either mock-treated (lanes 1–4) or supplemented with the protein synthesis inhibitor cycloheximide (CHX) at 10 μg/ml (lanes 5–8) or100 μ g/ml (lanes 9–12). After five minutes (time = 0), the cultures were released from cell cycle arrest by downshift to 25°C. At indicated time points, samples were withdrawn, and the levels of Clb2, Sic1 and Cdc28 proteins were assayed by SDS-PAGE and immunoblotting.

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