Figure 4

net1^tab2-1 efficiently bypasses multiple deletion mutants of MEN and extends the permissive temperature range for cdc15-2, cdc14-1, and cdc5-1. (A) The bypass specificity of net1^tab2-1 (net1-1). (B) Cells with specified combinations of NET1 alleles (indicated at the top) and CDC alleles (indicated on the side) were spotted onto YPD plates in 5-fold serial dilutions. Plates were incubated at 24° (left panel) or 32.5° (center and right panels) for 2–4 days before being photographed. net1^tab2-1 was observed to suppress the ts phenotype of dbf2-1 at 35.5°C (not shown).