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Table 2 Leu+ reversions under selection were cell density-dependent

From: Occurrence of leu+ revertants under starvation cultures in Escherichia coli is growth-dependent

 

Description

Number of micro-drops or plates with Ni papillae

 

Day 2

Day 4

Day 7

Day 11

Day 15

Day 18

A

8.27 × 107 cells/micro-drop

N0 = 589

N0 = 501

N0 = 475

N0 = 462

N0 = 344

N0 = 264

 

Total micro-drops = 620

N1 = 29

N1 = 108

N1 = 125

N1 = 116

N1 = 199

N1 = 246

 

Total cells = 5.13 × 1010

N2 = 2

N2 = 11

N2 = 13

N2 = 20

N2 = 49

N2 = 63

    

N3 = 7

N3 = 9

N3 = 23

N3 = 31

     

N4 = 3

N4 = 5

N4 = 13

       

N5 = 3

 

Papillae/micro-drop

0.053

0.210

0.277

0.315

0.619

0.858

B

8.27 × 107 cells/plate

N1 = 6

N1 = 8 N2 = 1

N1 = 8

N1 = 8

N1 = 7

N1 = 7

 

Total plates = 50

  

N2 = 2

N2 = 2

N2 = 2

N2 = 2

 

Total cells = 4.20 × 109

    

N3 = 1

N3 = 1

 

Papillae/plate

0.12

0.20

0.24

0.24

0.28

0.28

C

8.27 × 106 cells/micro-drop

N0 = 1600

N0 = 1600

N0 = 1600

N0 = 1599

N0 = 1598

N0 = 1598

 

Total micro-drops = 1600

   

N1 = 1

N1 = 2

N1 = 2

 

Total cells = 1.32 × 1010

      
 

Papillae/micro-drop

0

0

0

0.001

0.001

0.001

D

8.27 × 107 cells/micro-drop

N0 = 160

N0 = 154 N1 = 6

N0 = 152

N0 = 145

N0 = 137

N0 = 127

 

Total micro-drops = 160

  

N1 = 8

N1 = 15

N1 = 23

N1 = 33

 

Total cells = 1.32 × 1010

      
 

Papillae/micro-drop

0

0.038

0.05

0.094

0.144

0.206

E

2.96 × 108 cells/micro-drop

N0 = 45

N0 = 41

N0 = 33

N0 = 21

N0 = 11

N0 = 8

 

Total drops = 125

N1 = 38

N1 = 39

N1 = 42

N1 = 40

N1 = 45

N1 = 42

  

N2 = 24

N2 = 24

N2 = 27

N2 = 37

N2 = 40

N2 = 38

  

N3 = 11

N3 = 12

N3 = 13

N3 = 12

N3 = 13

N3 = 19

  

N4 = 6

N4 = 7

N4 = 8

N4 = 12

N4 = 11

N4 = 12

  

N5 = 1

N5 = 2

N5 = 2

N5 = 3

N5 = 5

N5 = 6

 

Papillae/micro-drop

1.18

1.29

1.42

1.70

1.90

2.02

F

2.96 × 108 cells/micro-drop

N0 = 125

N0 = 125

N0 = 125

N0 = 125

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Total drops = 125

      
 

Papillae/micro-drop

0

0

0

0

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  1. A,C,E: Cells were deposited as micro-drops on MM+P plates. B: Cells were spread on MM+P plates. D: cells were pre-cultured in 0.85% NaCl solution for 14 hours at 37°C before deposited as micro-drop on MM+P plates. F: Cells were deposited as micro-drops on MM+P plates with 100 μg/ml Ampcillin. Ampicillin was added once a day with 1 ml (1000 μg/ml) around the every micro-drops till day 7.