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Table 3 Leucine leaked from scavenger bacteria supporting the cells' growth and mutation

From: Occurrence of leu+ revertants under starvation cultures in Escherichia coli is growth-dependent

Number of cells per culture at starting time

Number of leu + revertants in different media

Time of scavenger bacteria culturing (day)

0

6 hours

24 hours

48 hours

120 hours

168 hours

2.89 × 105

0

0

0

0

0

0

2.89 × 106

0

0

0

0

0

0

2.89 × 107

0

0

0

1

2

1

2.89 × 108

0

1

1

5

4

5

2.89 × 109

0

2

3

17

21

17

Total revertants

0

3

4

23

27

25

CFUA/culture

2.31 × 105

1.36 × 106

3.54 × 106

8.53 × 106

3.73 × 106

3.02 × 106

CFUB/culture

7.64 × 107

1.69 × 108

3.23 × 108

3.69 × 108

4.46 × 108

4.10 × 108

Leucine (ng/ml)

------

578.7

842.3

971.9

963.2

962.1

  1. A: The number of viable cells of 31343 which were cultured in the medium at 37°C for 24 hours. The initial number of 31343 cells were 2.89 × 105 cells/ml. The medium was MM+P in which the scavenger bacteria cells (in the dialysis tubings) of 35577 were pre-cultured for different days at the concentration of 7.97 × 108 cells/ml. B Same as A except that the initial number of 31343 cells was 2.89 × 108 cells/ml.