Table 1 Strains used in this work.

AX2

NC4

Wild type (axenic)

[34]

AX3

NC4

Wild type (axenic)

[35]

DH1

AX3

pyr 56^-

[18]

JH10

AX3

thy A^-

[19]

IR110

AX3

thy A::G418^r

this work

IR150

AX2

thy A::G418^r

this work

DIR1

DH1/JH10

pyr 56^+/-, thy A^+/-

this work

DIR2

AX3/IR150

thy A⁺/thy A::G418^r

this work

JK41

AX3

ras S^-, gef B^-

this work

JK42

AX3

ras S^-, gef B^-

this work