Table 2 Oligonucleotide primers used to amplify ATOX 1 coding sequences and splice junctions
From: Genomic organization of ATOX1, a human copper chaperone
Exon | Forward Primer (5'-3') | Reverse Primer (5'-3') | Fragment Size |
|---|---|---|---|
Exon 1 | aggcgctgctgacaccgccg | ttcaagatcagcatccggtc | 151 bp |
Exon 2 | aggcttctgatgagtctgatgc | tctgcatgcatctgaacatg | 273 bp |
Exon 3 | tgagtagtaatttagagcctg | aggtgttcgctctgatgagag | 327 bp |