Figure 10
From: Fission yeast Rad26 responds to DNA damage independently of Rad3
Rad26.T12-GFP spots, but not speckles, form after Bleomycin treatment. Cultures were grown at 30°C in liquid, complete media to O.D. 0.3 and then treated with 5 mU/ml of Bleomycin for 3 hours. Cells were then prepared for microscopy following the Triton X-100 extraction method or the paraformaldehyde fixation method (see Methods). A. Spots and speckles form in Bleomycin-treated, Triton X-100 extracted rad26-GFP cells. rad26-GFP (TE1197) B. Spots, but not speckles, form in Bleomycin-treated, Triton X-100 extracted rad26.T12-GFP cells. rad26.T12-GFP (TE1206) C. Spots, but not speckles, form in paraformaldehyde-fixed rad26.T12-GFP cells. rad26-GFP (TE1197) and rad26.T12-GFP (TE1206) Bar = 5 μm