Figure 9
From: Fission yeast Rad26 responds to DNA damage independently of Rad3
Rad26.T12-GFP spots form in Bleomycin-treated rad26.T12-GFP cells. Cultures were grown at 30°C in liquid, complete media to O.D. 0.3 and then treated with 5 mU/ml of Bleomycin for 3 hours. Cells were then prepared for microscopy following the Triton X-100 extraction method (see Methods). This experiment was repeated three times with similar results, one of which is shown. rad26-GFP (TE1197) and rad26.T12-GFP (TE1206)