Figure 2

The confirmation of Ndrg1 protein induction in different cell lines. (A) HTE cells were incubated with different concentrations of NiCl₂ (Ni), hypoxia, and 300 μM of CoCl₂ (Co) for 20 hrs. (B) HOS and MCF-7, (C) PW and DU-145 cells were incubated with 500 μM of NiCl₂ (nickel), hypoxia, and 300 μM of CoCl₂ (cobalt) for 20 hrs. Western blot analyses were done as described in 'Methods' section. The membranes were first incubated with anti-Ndrg1 antibody (top panels), then stripped, and rehybridized with anti-actin antibody (bottom panels) to show loading.