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Figure 6 | BMC Genetics

Figure 6

From: The Prader-Willi syndrome murine imprinting center is not involved in the spatio-temporal transcriptional regulation of the Necdin gene

Figure 6

Mapping of DNase I hypersensive sites in the Ndn and Magel2 genomic region. (A) The diagram shows a map of the sequenced genomic region present in the BAC109. Localisation of HS sites is indicated by arrows in relation to the Ndn and Magel2 genes, and to the AK086725 EST. Position of Bgl II and BamH 1 sites and probes 1 to 10 are indicated. Asterics indicate polymophic Bgl II restriction sites present on castaneus alleles detected with probes 1/2 and probes 7/8. A, B and C design three non-coding regions of homology between the mouse and the human genomes.(B) Southern blot analysis of Bgl II or BamH I cut genomic DNA isolated from brain nuclei treated with increasing concentrations of DNase I (from 0 to 100 U). Maternal (mat) and paternal (pat) alleles are indicated when discrimination is possible.(C) Sequence comparison between mouse and human Ndn/Magel2 genomic regions. Alignments were peformed with the genome VISTA program (window size 100 bp, homology threshold 70%). Conserved non-coding sequences (CNS) are depicted in pink, untranslated regions (UTR) and ORF in pale and dark blue respectively. Genes are indicated by blue arrows. Repeated elements are depicted above the sequences comparison. Mapped HS sites are indicated by vertical arrows. A, B and C design three non-coding regions of homology between the mouse and the human genomes. Although the C region is depicted as a non-gening sequence, recent isolation of ESTs corresponding to this region suggests that it might belong to the Magel2 transcription unit. The asterisk above the C region indicates the beginning of the ESTs.

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