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Table 1 HAP-sensitivity of the mutants selected in our screening.

From: Genome-wide screening for genes whose deletions confer sensitivity to mutagenic purine base analogs in yeast

Class of mutants

Gene or ORF name deleted

Functional group

Response HAP in spot tests†

Induced mutant frequency (×10-7) by HAP#

Survival in presence of HAP#

Wild type

  

-

400

100%

Class I: mutants hypersensitive to HAP

HAM1

DNA1

HM, HS

13000

17%

 

ADE12*

DNA

HM, HS

10000

30%

 

AAH1*

DNA

M, S

5500

30%

 

ADE2

DNA

M, S

4400

70%

II class: Mutants sensitive to mutagenic effect of HAP

VIP1

Cell2

M, S

840

76%

 

VID27

Metabolic3

M, S

600°

82%

 

IPK1

Metabolic

M, S

1400

100%

 

ADE5,7

DNA

M

1900

100%

 

ADE8

DNA

M

1500

100%

 

ADE6

DNA

M

1100

100%

 

RIM101

meiosis

M

1100

100%

 

ADE3

DNA

M

940

100%

 

ADE1

DNA

M

860

100%

 

YGR035c

unknown

M

500°

100%

 

Yjl055w*

Metabolic

M

500°

100%

Class III: Mutants sensitive to killing

YMl013c-a

Unknown

S

230°

60%

 

SHE4

Cell

S

600°

30%

 

TRP2

Metabolic

HS

250°

60%

  1. †- HM – hypermutable, M – more mutable than wild-type, HS – hypersensitive, S – more sensitive than wild-type, see first paragraph od the Results Section for the explanation.
  2. # – 25 μg/ml
  3. 1 – 'DNA' – includes genes involved in the control of DNA precursor metabolism, purine salvage, DNA repair.
  4. 2 – 'Cell' – includes genes involved in cytoskeleton organization, cell walls and organelles.
  5. 3 – 'Metabolic' – includes genes involved in general metabolic pathways.
  6. * – These mutants were also sensitive to the mutagenic or toxic effect of AHA (see Fig. 3).
  7. ° – small difference from the wild-type strain was reproducible and statistically significant by Wilcoxon-Mann-Whitney test.