Table 6 PCR Primers used for the MLPH cDNA amplification and/or genomic MLPH mutation analysis

MLPH cDNA, first round amplification

Mlph_cDNA_Ex1_F1

CCT TCC TTC CCC TGT AGG AC

513 bp

52°C

Mlph_cDNA_Ex4_R

GGA TCA CCT TGG CAC TCC

Mlph_cDNA_Ex4_F1

GTG AAG ATC GGC TCG GTA G

686 bp

52°C

Mlph_cDNA_Ex9_R

GGA TGC TGA GAG GTG GTG

Mlph_cDNA_Ex7_F1

CTT GGA CTT TGA GGC AGA C

985 bp

52°C

Mlph_cDNA_Ex14_R

ACT GAA CTT CCT TCT GAG G

Mlph_cDNA_Ex10_F

AGA GAA GAG GAG ACC CTC

651 bp

52°C

Mlph_cDNA_Ex16_R

GCT GGG TCA TCA CAG GC

MLPH cDNA, second round amplification

Mlph_cDNA_Ex1_F2

CTG TAG GAC CGG AGA GAG C

502 bp

52°C

Mlph_cDNA_Ex4_R

GGA TCA CCT TGG CAC TCC

Mlph_cDNA_Ex4_F2

AGA TCG GCT CGG TAG AGT G

679 bp

52°C

Mlph_cDNA_Ex9_R

GGA TGC TGA GAG GTG GTG

Mlph_cDNA_Ex7_F2

CTC TGA CGA CTC CAC TTG

967 bp

52°C

Mlph_cDNA_Ex14_R

ACT GAA CTT CCT TCT GAG G

Mlph_cDNA_Ex10_F

AGA GAA GAG GAG ACC CTC

651 bp

52°C

same primers as in first round amplification

Mlph_cDNA_Ex16_R

GCT GGG TCA TCA CAG GC

MLPH genomic DNA

P_Mlph_Ex1_for1

AGT GGC CTC AAG CCC TG

591 bp

56°C

P_Mlph_Ex1_rev

ATG AGC TCC CTG AGA ACC

P_Mlph_Ex1_for2

CTC CTC CCG AGG CTC CTG

563 bp

56°C

P_Mlph_Ex1_rev

ATG AGC TCC CTG AGA ACC

Mlph_Ex2_for

GTC ACC GAC ATT ATG TCA CAG

598 bp

58°C

Mlph_Ex2_rev

CAG GCT GGA AGG TCA GAT C

Mlph_Ex3&4_for

GAG ACC CAG GAT CGA GTC

725 bp

56°C

Mlph_Ex3&4_rev

CAC TCA CAT TCC AAA GGA TC

Mlph_Ex5_for

AGA AGT GAT GGA GGT CAC TG

471 bp

56°C

Mlph_Ex5_rev

ACC GAA CAG GAA CAG GAG

Mlph_Ex6_for

AG CTT GCC TGG ATG GAT G

582 bp

53°C

Mlph_Ex6_rev

CTG CAG CCT CTG CCA AC

Mlph_Ex7_for

GTC CTC AGC ACT TCT GAG

568 bp

53°C

Mlph_Ex7_rev

GTG AGA AGC TTC TGG ACC

Mlph_Ex8_for

CAG CGG GAT TTC TGA AAG C

483 bp

56°C

Mlph_Ex8_rev

GTG TTG GAC AGT CAG AGT G

Mlph_Ex9_for

CCC GCC TTT GCC TTA AGC

416 bp

56°C

Mlph_Ex9_rev

GCT GCA AGG AGG AGC TTC

Mlph_Ex10_for

CAG AGC CTG GCT CCT GAG

600 bp

56°C

Mlph_Ex10_rev

CAC CTG GGA CAG GGA AGC

Mlph_Ex11_for

AGG CGT CCA GAG GTT GTG

550 bp

55°C

Mlph_Ex11_rev

GCC TGC TTC TGG AGA AGC

Mlph_Ex12_for

CGC CGA CCA AGT CTT TGC

623 bp

55°C

Mlph_Ex12_rev

TGG ACT TGA GGC CGT GTG

Mlph_Ex13_for

CCT GTC TCC CCA GAT TCG

539 bp

55°C

Mlph_Ex13_rev

GGG TTT GCC AAG GCT GAG

Mlph_Ex14_for

CTC CTT TAT GCT CTG GCA C

591 bp

55°C

Mlph_Ex14_rev

TTG TCA CAC GGA GAC ACA G

Mlph_Ex15_for

CTC AGG CAG GCA GAC AAG

461 bp

55°C

Mlph_Ex15_rev

TGC TCT GGG GTC CTA ACG

P_MLph_Ex16_for

GCT TCA GAG CCT GAA ATT CT

469 bp

53°C

P_Mlph_Ex16_rev

GAC AAA AGA TCA GGC TGG AG