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Table 1 Designed species-specific and selected universal oligonucleotide primers used in this study along with amplicon size.

From: Development of novel heminested PCR assays based on mitochondrial 16s rRNA gene for identification of seven pecora species

Species

Primer

name

Primer

direction

Primer

size (bp)

Primer

sequence

Species – Specific

amplicon size

All

16s Fwd

Forward

20

5'-CGCCTGTTTATCAAAAACAT-3'

 

All

16s Rev

Reverse

20

5'- CTCCGGTTTGAACTCAGATC-3

~550 bp

Antilope. Cervicapra

BB16

Reverse

21

5'-ACCTAGTTATTCGCTATCAAG -3'

410 bp

Naemorhedus. goral

GL16

Reverse

29

5'-GGCAACTAGTTCAAAAAACCTAGTTATTT -3'

430 bp

Boselaphus. tragocamelus

NG16

Reverse

24

5'-AGTTATTCGCTATCTCACTAAACC -3'

408 bp

Cervus. eldi

TH16

Reverse

22

5'-CCTAGTTATTTGCTAACACACC -3'

400 bp

Cervus. axis

CH16

Reverse

25

5'-TATCCCAGAAGGACAGAATAATTAC -3'

240 bp

Cervus. unicolor

SM16

Forward

24

5'-GCTTTAACTACTTAGCCCAAAGAG -3'

350 bp

Axis. porcinus

HD16

Reverse

27

5'-CTCACAACAACAAAGGAATCGCTACTC -3'

300 bp