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Table 1 Designed species-specific and selected universal oligonucleotide primers used in this study along with amplicon size.

From: Development of novel heminested PCR assays based on mitochondrial 16s rRNA gene for identification of seven pecora species

Species Primer
name
Primer
direction
Primer
size (bp)
Primer
sequence
Species – Specific
amplicon size
All 16s Fwd Forward 20 5'-CGCCTGTTTATCAAAAACAT-3'  
All 16s Rev Reverse 20 5'- CTCCGGTTTGAACTCAGATC-3 ~550 bp
Antilope. Cervicapra BB16 Reverse 21 5'-ACCTAGTTATTCGCTATCAAG -3' 410 bp
Naemorhedus. goral GL16 Reverse 29 5'-GGCAACTAGTTCAAAAAACCTAGTTATTT -3' 430 bp
Boselaphus. tragocamelus NG16 Reverse 24 5'-AGTTATTCGCTATCTCACTAAACC -3' 408 bp
Cervus. eldi TH16 Reverse 22 5'-CCTAGTTATTTGCTAACACACC -3' 400 bp
Cervus. axis CH16 Reverse 25 5'-TATCCCAGAAGGACAGAATAATTAC -3' 240 bp
Cervus. unicolor SM16 Forward 24 5'-GCTTTAACTACTTAGCCCAAAGAG -3' 350 bp
Axis. porcinus HD16 Reverse 27 5'-CTCACAACAACAAAGGAATCGCTACTC -3' 300 bp