Skip to main content

Advertisement

Table 2 Results from species-specific heminested PCR assay with target and non target species for designed primers

From: Development of novel heminested PCR assays based on mitochondrial 16s rRNA gene for identification of seven pecora species

Species Common Name DNA source 1st PCR universal primer 16s rRNA gene 2nd PCR BB 16 2nd PCR GL 16 2nd PCR NG 16 2nd PCR TH 16 2nd PCR CH 16 2nd PCR SM 16 2nd PCR HD 16
Antilope cervicapra a Blackbuck Tissue + + - - - - - -
Antilope cervicapra b Blackbuck Tissue + + - - - - - -
Antilope cervicapra c Blackbuck Blood stain + + - - - - - -
Boselaphus tragocamelus a Nilgai Tissue + - - + - - - -
Boselaphus tragocamelus b Nilgai Tissue + - - + - - - -
Boselaphus tragocamelus c Nilgai Tissue + - - + - - - -
Naemorhedus goral a Goral Blood stain + - + - - - - -
Naemorhedus goral b Goral Tissue + - + - - - - -
Naemorhedus goral c Goral Tissue + - + - - - - -
Axis porcinus a Hog deer Tissue + - - - - - - +
Axis porcinus b Hog deer Tissue + - - - - - - +
Cervus axis a Chital Blood stain + - - - - + - -
Cervus axis b Chital Tissue + - - - - + - -
Cervus axis c Chital Tissue + - - - - + - -
Cervus axis d Chital Tissue + - - - - + - -
Cervus axis e Chital Tissue + - - - - + - -
Cervus eldi a Thamin Tissue + - - - + - - -
Cervus eldi b Thamin Tissue + - - - + - - -
Cervus unicolor a Sambar Tissue + - - - - - + -
Cervus unicolor b Sambar Tissue + - - - - - + -
Cervus duvaucelii Barasingha Tissue + - - - - - - -
Ovis aries Sheep Tissue + - - - - - - -
Bos tarus Cow Tissue + - - - - - - -
Homo sapiens Human Blood + - - - - - - -
Lepidochelys olevacea Turtle Tissue + - - - - - - -
Hemidactylus fluviviridis Lizard Tissue + - - - - - - -
  1. + positive amplification, - negative amplification