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Table 1 Pairwise interaction tests between normal and mutant PAX6 constructs and HOMER3, DNCL1 and TRIM11. +++: strong interaction; ++: moderate interaction; +: weak interaction; (+) borderline interaction with one or two reporters activated at very low levels; 0: no interaction. PAX6PST, PAX6 PST domain; PAX6CTP, PAX6 C-terminal peptide; PAX6PST-CT, PAX6 PST domain minus the C-terminal peptide; PAX6PST/Q422R, PAX6 PST domain with the Q422R mutation; PAX6PST/X423L, PAX6 PST domain with the X423L mutation, PAX6PST/1615del, PAX6 PST domain with the 1615 del10 mutation. HOMER3-FL, HOMER3 full-length clone; HOMER3-Tr, truncated HOMER3 clone lacking the N-terminal 70 amino acids.

From: A screen for proteins that interact with PAX6: C-terminal mutations disrupt interaction with HOMER3, DNCL1 and TRIM11

pDBLeu constructs pPC86 constructs
  HOMER3-FL HOMER3-Tr DNCL1 TRIM11
PAX6PST +++ ++ ++ ++
PAX6CTP 0 0 0 0
PAX6PST-CT (+) 0 0 ++
PAX6PST/Q422R ++ + + ++
PAX6PST/X423L + 0 (+) ++
PAX6PST/1615del10 (+) 0 0 ++