Figure 4

Autoradiograph showing some of the SSCP gel phenotypes observed at the Uc3 locus. Three distinct patterns are evident, and sequencing confirmed that these correspond with three genotypes: "0606" (lanes 1, 5, 6, 10–14, 16, 18–20, 22 and 24), "0202" (lanes 2–4) and "0608" (lanes 15, 17, 21 and 23). Lanes 7–9 are too faint to score, and were rerun. SSCP successfully detected small sequence differences; alleles 02 and 06 differ by one substitution, alleles 06 and 08 differ by a 3-bp deletion. In this example, homozygotes display two bands in the upper ssDNA banding system (one band per complementary strand), while heterozygotes display four (but see Discussion). Here, the lower double-stranded DNA banding system is uninformative. Running conditions were 15 W for 4 h at 4°C, 40 cm gel.