Development and application of three-tiered nuclear genetic markers for basal Hexapods using single-stranded conformation polymorphism coupled with targeted DNA sequencing

Garrick, Ryan C; Sunnucks, Paul

doi:10.1186/1471-2156-7-11

Table 4 Population allele frequencies. Taxon and population abbreviations follow Table 1 and Figure 1, respectively. Allele frequencies for the Sm8 locus are based on the RFLP genotyping assay.

From: Development and application of three-tiered nuclear genetic markers for basal Hexapods using single-stranded conformation polymorphism coupled with targeted DNA sequencing

Taxon/locus		Population				Taxon/locus		Population
	Allele	HCR	ESR	PSR	BR		Allele	HCR	ESR	AR	PSR	BR
Pseud.						Acanth.
Sm2	N	68	79	106	61	Uc3	N	24	19	15	56	48
	A1	0.54	0.99	---	---		A1	---	---	---	0.02	---
	A2	0.08	---	0.39	---		A2	0.19	---	0.03	---	---
	A3	---	---	0.61	---		A4	---	---	0.23	---	---
	A4	---	---	---	0.90		A5	---	---	0.03	---	---
	A5	0.35	---	---	---		A6	0.81	1.00	0.27	0.76	0.52
	A8	---	---	---	0.02		A7	---	---	---	0.03	---
	A9	---	---	---	0.05		A8	---	---	0.10	0.04	---
	A10	---	---	---	0.02		A9	---	---	---	0.15	---
	A11	---	---	---	0.02		A10	---	---	---	0.01	0.01
	A12	0.03	---	---	---		A11	---	---	---	---	0.04
	A13	---	0.01	---	---		A12	---	---	---	---	0.28
	A13	---	---	0.07	---	0.12
Sm8	N	68	79	106	61		A14	---	---	---	---	0.03
	A1	0.15	0.48	0.72	0.01		A15	---	---	0.13	---	---
	A2	0.85	0.02	0.28	---		A16	---	---	0.07	---	---
	A3	---	0.50	---	0.88		A17	---	---	0.07	---	---
	A4	---	---	---	0.12
Uc180	N	22	19	15	56	48
SmEF-1α	N	68	78	106	61		A1	---	---	1.00	---	---
	A1	0.07	---	0.07	---		A2	---	---	---	0.01	---
	A2	0.07	---	---	---		A3	---	---	---	---	0.01
	A3	---	---	0.05	---		A4	0.50	---	---	---	---
	A5	---	0.07	---	---		A5	0.18	---	---	---	---
	A6	0.85	0.89	---	---		A6	---	0.97	---	---	---
	A7	---	---	0.09	---		A7	---	0.03	---	---	---
	A8	---	0.05	0.34	---		A8	0.21	---	---	0.99	0.99
	A10	---	---	---	0.37		A9	0.11	---	---	---	center---
	A11	---	---	---	0.06
	A12	---	---	---	0.03	UcEF-1α	N	24	19	15	56	48
	A13	---	---	---	0.02		A1	---	0.03	---	0.59	---
	A14	---	---	---	0.01		A2	0.21	---	---	---	---
	A15	---	---	0.04	---		A3	0.52	0.92	---	---	---
	A16	---	---	---	0.17		A4	0.02	0.03	---	---	---
	A17	---	---	---	0.02		A5	---	---	1.00	---	---
	A18	---	---	0.41	---		A6	0.15	---	---	---	---
	A19	---	---	---	0.33		A7	---	---	---	0.16	---
	A20	0.01	---	---	---		A8	---	---	---	0.02	---
	A21	---	---	---	0.01		A9	---	---	---	0.13	0.06
	A10	---	---	---	0.01	---
Sm6	N	68	79	106	61		A11	---	---	---	0.01	---
	A1	1.00	0.60	0.35	---		A12	---	---	---	---	0.81
	A2	---	---	---	0.07		A13	---	---	---	0.08	---
	A3	---	0.41	0.04	---		A14	---	---	---	---	0.09
	A4	---	---	0.61	0.84		A15	0.02	---	---	---	---
	A5	---	---	---	0.09		A17	---	---	---	---	0.02
	A19	---	0.03	---	---	---
Sm4	N	68	79	106	60		A20	---	---	---	---	0.01
	A1	---	0.35	---	0.08		A21	0.08	---	---	---	---
	A2	---	0.21	0.09	---
	A3	1.00	0.44	0.91	0.88	UcANT	N	24	19	15	56	48
	A4	---	---	---	0.03		A1	1.00	1.00	---	0.55	---
	A2	---	---	---	0.45	1.00
Sm150	N	68	79	106	61		A3	---	---	1.00	---	---
	A1	1.00	1.00	0.08	1.00
	A2	---	---	0.93	---	UcWnt	N	24	19	15	56	48
	A1	1.00	1.00	1.00	0.24	0.27
							A2	---	---	---	0.18	0.03
							A3	---	---	---	0.58	0.70
						Uc44	N	24	19	15	56	48
							A1	0.71	0.32	0.03	---	---
							A2	---	0.03	0.57	---	0.01
							A3	0.08	0.37	---	---	---
							A4	---	---	---	0.01	---
							A5	0.21	0.29	0.40	0.99	0.97
							A6	---	---	---	---	0.02

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