Table 3 Primer sequences for the amplification of canine CLCN3 cDNA
From: Characterization of the canine CLCN3 gene and evaluation as candidate for late-onset NCL
Primer | Sequence (5' – 3') | Localization within canine CLCN3 | TM(°C) |
|---|---|---|---|
5' RACE outer primer | TGTACGAGCCAGGACCTTCT | exon 4/exon 5 junction | 60 |
5' RACE inner primer | TTTGTCATTTCCCATGCTGA | exon 2 | 60 |
3' RACE outer primer | TGCTTTAGTGGCTGCATTTG | exon 8 | 60 |
3' RACE inner primer | TGACTGTCTCCCTGGTGGTT | exon 10 | 60 |
CLCN3_F1 | ATGGATGCTGCTTCTGATCC | exon 1 | 60 |
CLCN3_R10 | CAGCAGCCAGAGTGGTATGA | exon 10 | 60 |