Table 4 Primer sequences for the amplification of canine CLCN3 exons
From: Characterization of the canine CLCN3 gene and evaluation as candidate for late-onset NCL
Forward primer | Sequence (5' – 3') | Reverse primer | Sequence (5' – 3') | TM(°C) | Product size (bp) |
|---|---|---|---|---|---|
CLCN3_Ex1_F | AGCAGGGGTGGAAGAAATG | CLCN3_Ex1_R | AACTACAGAACCGCCCAGC | 60 | 233 |
CLCN3_Ex2_F | ACCTAGTTCACCATTGTCTCTCA | CLCN3_Ex2_R | TATTTTGGCTGCCAGAGGTC | 60 | 312 |
CLCN3_Ex3_F | ACCCCTTGCTCTCAAATCCT | CLCN3_Ex3_R | TTGTAGGGTGAAGGAGAGAACT | 60 | 418 |
CLCN3_Ex4_F | GTCTCAACACTCCAAAAGTGGAC | CLCN3_Ex4_R | CTGTAATTAAACGGAGACTCATCTCA | 60 | 321 |
CLCN3_Ex5_F | TGTGGAAGTAAGCCAAGAAACTC | CLCN3_Ex5_R | CTCCCCCTAAAGGCAAAAAG | 60 | 318 |
CLCN3_Ex6_F | AAGTGTTCCTGTTTCCTGAATGA | CLCN3_Ex6_R | GACTGAGCAGTACTGGGGATG | 60 | 459 |
CLCN3_Ex7_F | TTGGAAAGAGGTAGCCATCG | CLCN3_Ex7_R | GGCTTTTCTCAAGGTAAAGAACAT | 60 | 936 |
CLCN3_Ex8_F | GCTGCAGCAAAAATTAGACCA | CLCN3_Ex8_R | AAATGGAACCCAAAAGATAAGAA | 60 | 781 |
CLCN3_Ex9_F | AGTTTTATTTGTACTAGGATTTTGCTC | CLCN3_Ex9_R | CAATAGCAGTACTGTTTCATTTCTGTG | 60 | 474 |
CLCN3_Ex10_F | TCCTGTCCTCCTTGACCAAT | CLCN3_Ex10_R | CCCCCAGAAACCCAACTAAT | 60 | 579 |
CLCN3_Ex11_F | GGGACCAAATTCATGGGATA | CLCN3_Ex11_R | TGTTTTGGCAAAGATGTGGT | 60 | 511 |
CLCN3_Ex12_F | GGACCTGGGATTTCGAACC | CLCN3_Ex12_R | TTATTCAGCAGGCATCTGGG | 60 | 343 |
CLCN3_Ex13_F | ATCAAAGGATGGTTGCTGGA | CLCN3_Ex13_R | TTGCGATGTCGGAGTAACAG | 60 | 647 |