Figure 3

Genotyping of the 10 markers of CYP7A1 htSNPs and small deletion on thin-film biosensor chips. A. A design for arraying the capture probes on a thin-film biosensor chip. A pair of capture probes for each SNP were arrayed in duplicate next to each other with allele 1 left and allele 2 right. M indicates a positive control marker with 20 dATP and 3'-biotin. B. SNP discrimination with a pool of synthetic oligonucleotide targets of allele 1. C. SNP discrimination with a pool of synthetic oligonucleotide targets of allele 2. D. Negative control with no targets. E. A representative image showing genotypes of a Mexican-American individual with homozygous allele 1 for the most markers, but homozygous allele 2 for rs8192879. F. A representative image showing genotypes of a Mexican-American individual with homozygous allele 2 for the most markers, but homozygous allele 1 for rs18192879, rs11786580, rs8192874, 1302 TT. G. A representative image showing genotypes of a Mexican-American individual with heterozygous for the most markers except rs8192874, 1302 TT.